To elucidate the transcriptional response of a natural bacterial community to dissolved organic matter produced by a phytoplankton bloom, a microcosm experiment was performed. Coastal seawater from Gulf of Mexico was collected and dispensed into microcosms. Two replicate cubitainers were amended with nutrients (N and P) to stimulate phytoplankton bloom, while two untreated cubitainers served as controls. The bacterial community metatranscriptome (0.22 - 5um size fraction) was sequenced from nutrient amended microcosms and untreated controls on day 5, while the phytoplankton bloom had reached its peak.
Dauphin Island Cubitainer Experiment (DICE)
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