Karenia brevis CCMP2229

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Environmental Data
Principle Investigator(s)	Geoff Sinclair, Boris Wawrik
External sample ID	K. brevis L1 - P
NCGR Sample ID	MMETSP0030
Sample accession number	CAM_SMPL_002358
Assembly accession number	CAM_ASM_000176
Combined Assembly Name	Karenia-brevis-CCMP2229
Genus	Karenia
Species	brevis
Strain	CCMP2229
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	156230
18S rRNA	TCCACTGCAACGTTCAGGATCTGAACACTGCGGCAGTCCGAATTATTCACCGGATCACTCAATCGGTAGGAGCGACGGGC GGTGTGTACAAAGGGCAGGGACGTAATCAGCACGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTGAAGATTAATA ATTGCAATAATCTATCCCCATCACGATGCGTTTTAAAAAGATTACCCAACCCTTCCGGGCAAGGTCGAAAACTCGTTGAG CGCATCAGTGTAGCGCGCGTGCAGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGCGTTA GACACGCAAAGTCCCTCTAAGAAGTTGCCCACGTAACCGAAGTTACGTGTAACTATTTAGCAGGTTAAGGTCTCGTTCGT TAACGGAATTAACCAGACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTAT CAATCTGTCAATCCTTACTATGTCTGGACCTGGTAAGTTTCCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTG GTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCA TAAGGTGCTGAAGGAGTCGTATAAGTAACGACCTCCAATCTCTAGTCGGCATGGTTTATGGTTAAGACTAGGACGGTATC TGATCGTCTTCGATCCCCTAACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCACAGTAGTCCGTCTTT AACAAATCCAAGAATTTCACCTCTGACAGTTAAATACGAATGCCCCCAACTATCCCTATTAATCATTACCTCAGCTCTAG AAACCAACAAAATAGAACCGAGGTCCTATCTTATTATTCCATGCTAATGTATTCAAGGCGTATGCCTGCTTGAAACACTC TAATTTCCTCAAAGTAAAAGTCCTGGATACCGCACCACACAGTCAAGTGCAGCTACGTTCTCCAAGAAGATGCCCAAGCC GAGCCAGATACTCACCCAGAGGGCGGACCGGTCGTCCTCGGCAGAAATCCAACTACGAGCTTTTAACCGCAACAACTTTT ATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGATACTCGCAAAGGGATTTAA ATTCTACTCATTCCAATTACAAGACATGGATGCCCTGTATTGTTATTTCTTGTCACTACCTCCCTGTGTCAGGATTGGGT AATTTGCGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCG TTACCCGTCAATGCCACGGTAGGCCAATACCCTACCGTCGGAAACTGATAGGTCAGAAACTTGAATGATCTATCGCCAGC TGATGCAATGCGATTCGTTCGGTTATTATGAATCACCACAAGACCAGGCAGGGCCTGGGTTGGTTCTGTATCTAATAAAC ACAACCCTTCCAAGAAGTCGGGTTTGGGCGCATGTATTAGCTCTAGAATTTCCAAAGTTATCCATGTAATGAATGACCAT CAAATAAACTATTACTGTTTTAATGAGCCATTCGCAGTTTCGCCGTATAGAAGCTTATACTGA
Importance of organism and transcriptomes	Dinoflagellates are a key component of the eukaryotic phytoplankton in all of the world?s oceans. Despite their importance, mechanistic understanding of their physiological responses to important environmental changes, such as nutrient loading remains relatively poorly understood, since very little genetic information is available due to the immense size of their genomes. Transcriptome sequencing will provide significant insight into the genomic complexity of this group and a foundation upon which to explore the linkages between genomic and phenotypic responses in response to environmental changes that threaten coastal ecosystems.
Additional citations and references
Latitude	27.0171
Longitude	82.4763
Habitat description	coastal
Habitat	marine habitat
Experimental Data
Date of experiment	01-AUG-07
Growth medium	L1
Modifications to growth medium	none
Temperature (ºC)	22
Salinty (psu)	32
pH	8.2
Light (µmol photons / m2 / sec)	60
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	60
Trace elements (total) (nmol/L)	1170
Prey organism, if applicable (genus and species)	none
Other experimental metadata available	Isolated from near Manasota Florida at station VS05