Lotharella amoebiformis CCMP2058

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Environmental Data
Principle Investigator(s)	John Archibald
External sample ID	La1
NCGR Sample ID	MMETSP0042_2
Sample accession number	CAM_SMPL_002350
Assembly accession number	CAM_ASM_000168
Combined Assembly Name	N/A
Genus	Lotharella
Species	amoebiformis
Strain	CCMP2058
Clonal	Yes
Axenic	Yes
Prelim. NCBI Taxon ID	168855
18S rRNA	ATATGCTTGTCTCCAAGATTAAGCCATGCAAGTCTAAGTTTAAATAATTAACCTTGAAACTGCAGATGGCTCATTAAACCAGATTTAATTTATTTGATGGTGACTTTTTACATGGATACCTGTTTAAAATGAAGAGCTAATACATGCGTTAAACTCTGGCCTTTGGTCGGAGGTATTTATTAGATCCGAACCAGCCCCTTTTTGGGTTTTTTGGTGAATCATGATAACTGTTCGAATCGCCTATTTTCGGGCGATAGTTCATTCAAAAATCTGCCCTATCAGCTATTGACGGTAGTGTAGTGGACTACCGTGGCATTTACGGGTGACGGGAAATTAGGGTTTGATTCCGGAGAGGCAGCCTGAGAAACGGCTACCACATCCAAGGATAGCAGCAGGCGCGTAAACTATCTCCTGAAATACCTTCGAGATGGTGACAAGAAATATGATGACTGTGCCCTTTGCGGCAGAGTTTTTCAAATGAGTGTAATGTAAATGCCTATGCGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTTTCTTAACGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATATCTGGCTTTTTAGGTGGACGGCCCGCCTTCGGGTTGAGTGCTGGAAGCCTGAGCCTATTTTTCGGTTTGCGGTCTTTCCCTTTATTGTGGCGAGGCGTGCGATCCGGATCCGTTACCTTGAAAAAATCCGAGTGTTCAAAGCAGGCATTATTTTGCCGTGAAGATTGCAGCATGGAATAGCAACCATAGGACTTTAGCGTCTTATTCGTTGGTTCTGGGACCTAAGTAATGATTGATAGGGACAGTTGGGGGTGCTGCTATTCCGCGGCCAGAGGTGAAATTCTCAGATTCCCGGAAGAGCAACTTATGCGAAAGCATTCACCAAGGATGTCTTCTTTAATCAAGAACGAAAGTTGGGGGATCGAAGACGATCAGATACCGTCGT AGTCTCAACCATAAACTATTCCGACTAGGGATTGGTGGACGTTGACTTTTTATCCGACTTCATCAGCACCTTGTGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGACACCACCAGAAGTGGAACTTGCGGCTTAATTTGACTCAACACGGGAAAACTTTCCAGGTCCAGACATAGTGAGGATTGACAGATTGAAGATCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCGACCTGCTAAATAGTTGTGGGATGGCTTCGGGCAACCTTTTGTGTGCAGCCTCATAGAGGGACTATCGGACCCAACCCGGGTGGAAGTGCGAGGCAATAACAGGTCTGTGATGCCCATAGATGTTCTGGGCCGCACGCGTGATACTCAGATGCGTTTATCAAGTTTGTTTTGTCCTTGGCTCGAGAGGCCTGGGTAATCTTGAAATCGTATCGTGCTGGGGATAGATTATTGTAATTTTTAGTCTTGAACGAGGAATTTCTAGTAAACGCGAGTCATCAACTCGCATTGATTACGTCCCTGTCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATTATTTGGTGAGCGCCACGGACTGTTGCTGGCCTTTGCAAGAGGGTTGGCTTCTTGGGAAGTGGAACGAACCTGATGATTTAGAGGAAGTAAAAGTCGTAACAAGGTT
Importance of organism and transcriptomes	Although the chlorarachniophytes are not large group, they have attracted considerable attention for a number of reasons relating to their evolution and cell biology. Lotharella amoebiformis is the only species that a genetic transformation is available in the chlorarachniophytes. The transcriptomes are useful in cell biological analyses such as protein localizations.
Additional citations and references	DOI:10.1111/j.1440-1835.2010.00597.x; DOI:10.1111/j.1529-8817.2010.00851.x
Primary citation for organism's characterization, if available	Ishida K., Ishida N. and Hara Y. (2000) Lotharella amoeboformis sp. nov.: a new species of chlorarachniophytes from Japan. Phycological Research 48(4):221-229.
Latitude	26.45291
Longitude	127.80296
Collection date	01-OCT-89
Sample collection site	OTHER
Other collection site info	Moon Beach
Sample material (e.g. "seawater," "sediment," etc.)	sand
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Other
Habitat	marine habitat
Country	JAPAN
Experimental Data
Date of experiment	17-OCT-10
Growth medium	ESM medium
Temperature (ºC)	20
Salinty (psu)	35
pH	8
Light (µmol photons / m2 / sec)	55
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	1410
Phosphate (μmol/L)	29
Investigation type	Eukaryotes