Marine Microbial Eukaryote Transcriptome Sequencing Project
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Hemiselmis andersenii CCMP644
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| Principle Investigator(s) | John Archibald |
|---|---|
| External sample ID | Ha1 |
| NCGR Sample ID | MMETSP0043_2 |
| Sample accession number | CAM_SMPL_002351 |
| Assembly accession number | CAM_ASM_000169 |
| Combined Assembly Name | N/A |
| Genus | Hemiselmis |
| Species | andersenii |
| Strain | CCMP644 |
| Clonal | Unknown |
| Axenic | No |
| Prelim. NCBI Taxon ID | 464988 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Cryptomonads are aquatic unicellular eukaryotes that acquired photosynthesis through secondary endosymbiosis with a red algae. They are a key group to understand the origin and spread of photosynthesis among eukaryotes, but too few sequence data exist to properly address this question. The nucleomorph genome of H. andersenii has been sequenced, the nucleus transcriptome will nicely complement the existing data. |
| Additional citations and references | |
| Environmental Data | |
| Primary citation for organism's characterization, if available | Lane,C.E.& Archibald,J.M. (2006).J. Eukaryot. Microbiol. 53 (6), 515-521 |
| Sample collection site | Gulf of Mexico |
| Sample material (e.g. "seawater," "sediment," etc.) | net tow |
| Other environmental metadata available | Gulf Stream |
| Other environmental metadata available | Gulf Stream |
| Habitat | marine habitat |
| Country | UNITED STATES |
| Experimental Data | |
| Date of experiment | 01-OCT-10 |
| Growth medium | K medium |
| Modifications to growth medium | without SiO2 |
| Temperature (ºC) | 17 |
| Salinty (psu) | 30 |
| pH | 7.5 |
| Day portion of day:night cycle in hours | 6 |
| Night portion of day:night cycle in hours | 18 |
| Nitrate (μmol/L) | 882 |
| Ammonium (μmol/L) | 50 |
| Phosphate (μmol/L) | 10 |
| Total Fe (nmol/L) | 11.7 |
| Trace elements (total) (nmol/L) | 14.09 |