Bigelowiella natans CCMP 2755

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Environmental Data
Principle Investigator(s)	John Archibald
External sample ID	bn1
NCGR Sample ID	MMETSP0045_2
Sample accession number	CAM_SMPL_002372
Assembly accession number	CAM_ASM_000190
Combined Assembly Name	N/A
Genus	Bigelowiella
Species	natans
Strain	CCMP 2755
Clonal	Unknown
Axenic	Yes
Prelim. NCBI Taxon ID	753081
18S rRNA	CATATGCTTGTCTCAAAGACTAAGCCATGCATGTCTAAGTATAAACGACTTTGTACTGTGAAACTGCAGATGGCTCATTAAATCAGAAATAATTTCTTTGATGGTGAAAATCTACACGGATACCCGTAGTAATTCTAGAGCTAATACGTGCGTAAACTCCATTGGAGGTATTTATTAGATCCGAACCACCCCCTTCGGGGTTTTCTGGTGAATCATGATAACTGTTCGAATCGCCAGGCTCTGCCAGGCGATAGTTCATTCAAATTTCTGCCCTATCAGCTATTGACGGTAGTGTAGTGGACTACCGTGGCCGTAACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTGGTGACAAGAAATATCGTGACTGTGCCCTTTGTGGCAGAGTCTTCGAAATGAGCAATACGTAAATGCCTTTGCGAGTATCCATTGGAGGGTAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATATCTGGCTTTGCACGGACGGCCCGCCTTCGGGTTGAGTGCTGGAAGTGCTAGCCTATTTTCTGGTTGGCGTTCTTTCCCTTAATTGTGGCGAGTTTCGCGATCCGGATCGTTTACTTTGAAGAAATTCGAGTGTTCAAAGCAGGCTTACGCCGTGAAGATTGCAGCATGGAATAATGACATAGGACTATCGTGTTCTATTTTGTTGGTTCTGGGACCGTAGTAATGATTGATAGGGACAGTTGGGGGTGCTGCTATTCCGCGGCCAGAGGTGAAGTTCTCAGATTCCCGGAAGAGCAACTTATGCGAAAGCATTCACCAAGGATGTCTTCTTTAATCAAGAACGAAAGTAGGGGGATCGAAGACGATCAGATACCGTCGTAGTCTCTACCATAAACTATGCCGACTAGGGATTGGTGGACGTTGTTTTATCCGACTCCATCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGACACCACCAGAAGTGGAACTTGCGGCTTAATTTGACTCAACACGGGAAAACTTTCCAGGTCCAGACATAGTAAGGATTGACAGATTGAAGATCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCGACCTGCTAAATAGGTTCAGTATCGATGCGTCGATGCTTGGAGCTTCTTAGAGGGACTATCGGATCCAAACCGGTGGAAGTGCGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGTGTTACACTGACGCATTCATCAAGCGCCACACCTTGGCTCGAGAGGCCTCGGTAATCTTGAAAGTGCGTCGTGTTAGGGATAGATTATTGTAATTATTAGTCTTGAACGAGGAATTTCTAGTAAACGCGAGTCATCAACTCGCATTGATTACGTCCCTGTCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATCATTTGGTGAGGCTCACGGACTGTCGTGCTTCCTTCCTCGTGTTGGTTGTACTTCTTGGGAAGTGATACAAACCTGATGATTTAGAGGAAGTTAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAG
Importance of organism and transcriptomes	Bigelowiella natans is a chlorarachniophyte alga. Chlorarachniophytes are unusual among secondary plastid-containing algae in that they still possess the nucleus (nucleomorph) and cytoplasm of their algal endosymbionts in a highly reduced and simplified form. Investigating the nuclear genome of these organisms will improve our understanding of secondary endosymbiosis and the integration of their respective hosts and endosymbionts at the genetic, biochemical, and cellular levels. A better understanding of the relationships between chlorarachniophytes and other red and green algal secondary plastid-bearing organisms will also be possible.
Additional citations and references
Primary citation for organism's characterization, if available	Moestrup O, Sengco M. 2001. Ultrastructural studies on Bigelowiella natans, gen. et sp. nov., a chlorarachniophyte flagellate. J. Phycol. 37:624?46
Experimental Data
Date of experiment	22-JAN-11
Growth medium	f/2
Modifications to growth medium	f/2 without silicate
Temperature (ºC)	25
Salinty (psu)	35
pH	7.2
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Ammonium (μmol/L)	50
Phosphate (μmol/L)	10
Trace elements (total) (nmol/L)	122766