Guillardia theta CCMP 2712

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Environmental Data
Principle Investigator(s)	John Archibald
External sample ID	gt1
NCGR Sample ID	MMETSP0046_2
Sample accession number	CAM_SMPL_002373
Assembly accession number	CAM_ASM_000191
Combined Assembly Name	N/A
Genus	Guillardia
Species	theta
Strain	CCMP 2712
Clonal	Unknown
Axenic	Yes
Prelim. NCBI Taxon ID	905079
18S rRNA	TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTTAGTGTAAAA TAACTCTATTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATGGTTCCTTACTACATGG ATAACCGTAGTAATTCTAGAGCTAATACATGCACCAAGGCCCGACTCACGGAGGGTTGTATTTATTAGAT TCAAAACCAGCACCACCTGGTGATTCATAGTAACTAAACGAACCGCATGCCTTTATGGTGGCGGTGATTC ATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGAGGCCTACCATGGTTTTAACGGGTGACGGA GAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG CAAATTACCCAATCCCGACTCGGGGAGGTAGTGACAATAAATAACAATACAGGGCCAACGGTCTTGTTAT TGGAATGAGAACAATTTAAATCCCTTAACGAGGATCAATTAGAGGGCAAGTCTGGTGCCAGCAGCCGCGG TAATTCCAGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCGGAAGTCGGGCTCG GGCAGGCTGTCGGCCTCGGTTGGGCAGCTTGCTTGGGTCTTCTAGCTCGGGGGCCCCGTGCCATTTAACC GTGGTGCGCGGGAACACGGGCCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCTACGCTTGAAT ACATTAGCATGGAATAATGGAATAGGACTTTGGTGCTATTTCGTTGGTTAATGGGACCGAAGTAATGATT AAAAGGGACAGTTGGGGCCGTTTATATTTCGTTGTCAGAGGTGAAATTCTTGGATTTACGAAAGATAAAC TTCTGCGAAAGCATTCGGCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCA GATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCAGTGGATGTCAATTTGCGACGCCATT GGCACCTTGTGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGA ATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAG GTCCAGACATAGTAAGGATTGACAGATTGAAAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT TCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCAT CGCGAATATTGATTGGCGAGGGCTTCTTAGAGGGACTATTTGTGTTTAATGAATGGAAGTTTGAGGCAAT AACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGAACGCAACGAGCTCGA CCCTGAACCGAAAGGCCTGGGTAAACTTGTGAAAGTTCATCGTGATGGGGATAGATTATTGCAATTATTA ATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGATTACGTCCCTGCCCTTTGTAC ACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCCATTTGCCGCAAG GTGAGTGGTTGCGAGAAGTTGATTGAACCTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGT AGGTGAACCTGCGGAAGGATCAT
Importance of organism and transcriptomes	Guillardia theta is a cryptophyte alga. Cryptophytes are unusual among secondary plastid-containing algae in that they still possess the nucleus (nucleomorph) and cytoplasm of their algal endosymbionts in a highly reduced and simplified form. Investigating the nuclear genome of these organisms will improve our understanding of secondary endosymbiosis and the integration of their respective hosts and endosymbionts at the genetic, biochemical, and cellular levels. A better understanding of the relationships between cryptophytes and other red algal secondary plastid-bearing organisms will also be possible.
Additional citations and references
Habitat	marine habitat
Experimental Data
Date of experiment	25-NOV-10
Growth medium	h/2
Modifications to growth medium	h/2 without silicate
Temperature (ºC)	23
Salinty (psu)	35
pH	7.2
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Ammonium (μmol/L)	500
Phosphate (μmol/L)	10
Trace elements (total) (nmol/L)	122766