Pyramimonas parkeae CCMP726

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Environmental Data
Principle Investigator(s)	Aurora Nedelcu
External sample ID	Pyramimonas parkeae CCMP726
NCGR Sample ID	MMETSP0059
Sample accession number	CAM_SMPL_002366
Assembly accession number	CAM_ASM_000184
Combined Assembly Name	Pyramimonas-parkeae-CCMP726
Genus	Pyramimonas
Species	parkeae
Strain	CCMP726
Clonal	Yes
Axenic	Yes
Prelim. NCBI Taxon ID	36894
18S rRNA	GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCTGATTATACTGTGAAACTGC GAATGGCTCATTAAATCAGTTATAGTTTATTTGATGGTACTACTACTCGGATAACCGTAGTAATTCTAGA GCTAATACGTGCGCAACTCCCGACTTCTGGAAGGGACGTATTTATTAGATAAAAGACCAGCCGCCCTCGG GCGTTTTGTGGTGAATCATGATAACTTGTCGGATCGCATGGCCTTGTGCCGGCGACGTTTCATTCAAATT TCTGCCCTATCAACTTTCGATGGTAGGATAGAGGCCTACCATGGTGGTAACGGGTGACGGAGAATTAGGG TTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACC CAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATACCGGGCTTTTTCAAGTCTGGTAATTGGAAT GAGAACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTC CAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGATGGGAACGAC CGGTCCGCCGTTTCGGTGTGCACTGGACGATTCTATCTTGTTGTCGGGGACGCGCTCGTGGGCTTAACTG TCTGCGACGCGGAGTCGGCGGTGTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCAACCGCTCTGAAT ACATTAGCATGGAATAACGCTATAGGACTCTGGTCTTATTGTGTTGGTCTTCGAGACCGGAGTAATGATT AAGAGGGACAGTTGGGGGCATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAAC TTCTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCA GATACCGTCCTAGTCTCAACCATAAACGATGCCGACTAGGGATTGGCGGATGTTAAATCGATGACTTCGC CAGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGG AATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCA GGTCCAGACATAGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCG TTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAATAGTC CCACAAATTCTGAATTTGCGGCTGGCTTCTTAGAGGGACTATCGGCGTTTAGTCGATGGAAGTTTGAGGC AATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGAATTCAACGAGCT CCTAACCTAGGCTGAAGAGTCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATTATTGCAATTA TTAATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGATTACGTCCCTGCCCTTTG TACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAATGTTCGGACCGCTGCGAGGCGGACGG TCCGCCGTCAGCTTCGCGGTGGGAAGTTCATTAAACCTTATCATTTAGAGGAAGGAGAAGTCGTAACAAG GTTTCCGTAGGTG
Importance of organism and transcriptomes	Pyramimonas parkeae, a unicellular planktonic quadriflagellate, belongs to the Pyramimonadales, which is sister to the Mamiellales but also shares an unusual evolutionary history with euglenids. Although sexual reproduction has not been demonstrated in pyramimonadalean species, encystment is a well characterized feature of this group, suggesting that sex may occur. Overall, its phylogenetic proximity to the well-studied Mamiellales, its shared evolutionary history with euglenids, and the potential for sexual reproduction make P. parkeae (and the Pyramimonadales as a whole) an excellent candidate for transcriptome sequencing.
Additional citations and references
Primary citation for organism's characterization, if available	Pearson BR, Norris RE. 1975. Fine structure of cell division in Pyramimonas parkeae Norris and Pearson (Chlorophyta, Prasinophyceae). J Phycol. 11:113-124.
Latitude	36.64
Longitude	121.93
Collection date	13-APR-66
Other collection site info	Hopkins Marine Station, California
Sample material (e.g. "seawater," "sediment," etc.)	seawater (high tide pool)
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	01-JUL-11
Growth medium	f/2-Si
Modifications to growth medium	Prepare as for f/2 medium but omit Na2SiO 3 ? 9H2O.
Temperature (ºC)	20
Salinty (psu)	32
pH	8
Light (µmol photons / m2 / sec)	40
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	880
Phosphate (μmol/L)	36
Trace elements (total) (nmol/L)	24.5
Investigation type	Eukaryotes