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Undescribed Undescribed D1

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Principle Investigator(s) Brian Palenik
External sample IDCercozoanNOSYN
NCGR Sample IDMMETSP0086_2
Sample accession numberCAM_SMPL_002368
Assembly accession numberCAM_ASM_000186
Combined Assembly NameUndescribed-sp-D1
GenusUndescribed
SpeciesUndescribed
StrainD1
ClonalYes
AxenicNo
Prelim. NCBI Taxon ID136419
18S rRNA
Importance of organism and transcriptomesCercozoans are frequently found in 18S rRNA clone library surveys of marine environments and are thus considered ubiquitous in marine environments. They likely consume bacteria including cyanobacteria as prey, and thus are involved in recycling of nutrients through the microbial loop. Their biology is not well studied and we could not find a genome project for this group. This isolate was obtained from the SIO pier (UCSD, La Jolla, CA), has been in culture for more than a year and grows on a single Synechococcus strain and contaminating bacteria as food. This group would be important for phylogenetic reasons as part of a poorly sampled superfamily and for ecological reasons. We would hope to compare its transcriptome during growth with and without Synechococcus and to other nanoflagellates growing with and without Synechococcus.
Additional citations and references
Environmental Data
Primary citation for organism's characterization, if availableBass, D; Howe, AT; Mylnikov, AP et al. (2009) Phylogeny and Classification of Cercomonadida (Protozoa, Cercozoa): Cercomonas, Eocercomonas, Paracercomonas, and Cavernomonas gen. Nov. PROTIST Volume: 160 Issue:4 Pages:483-521
Experimental Data
Date of experiment15-MAR-11
Growth mediumf/4 amended seawater
Temperature (ºC)20
Salinty (psu)34
pH8
Light (µmol photons / m2 / sec)20
Day portion of day:night cycle in hours24
Nitrate (μmol/L)441
Phosphate (μmol/L)18
Trace elements (total) (nmol/L)12.25
Prey organism, if applicable (genus and species)Unknown bacteria