Undescribed Undescribed D1

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Environmental Data
Principle Investigator(s)	Brian Palenik
External sample ID	CercozoanWITHSYN
NCGR Sample ID	MMETSP0087
Sample accession number	CAM_SMPL_002395
Assembly accession number	CAM_ASM_000213
Combined Assembly Name	Undescribed-sp-D1
Genus	Undescribed
Species	Undescribed
Strain	D1
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	136419
18S rRNA	TATATCAGCAATTATTTATTTGATGGTCCCTTACTACATGGATAACTGTGGTAATTCTAGAGCTAATACATGCGTAAAATCTCGACTCTCGCGGGAAGAGATGTATTTATTAGATAAAAAACCAATGCGCCCTCGGGCGGTTTTGTGGTGAATCATAGTAACTTTTCGAATCGCATGGCTTCACGCTGGCGATAGTTCATTCAAATTTCTGCCCTATCAGCTTTCGACGGTAGTGTAGTGGACTACCGTGGCGTTAACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAAAAAATAACAAAATCGGGCATTTTCATGTCTGATTATTGGAATGAGAACAATTTAAATCACTTAACGAATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGCACGATGCGCCTGGCCCGCCTCATTACGAGTGTGTGTGCTAGTGCGCGTCTGCCATCCATTTACAGAACGGTCGTACCTTTCATTAGGCCGCGCTCGTGATGTAAATCTTTTACTTTGAAAAAATTAGAGTGTTTAAAGCAGGCATTTGCTATGAATACATTAGCATGGAATAATAATCTAGGACTCTGGTTCTATTTTGTTGGTTTCTAGTACTGGAGTAATGATTGATAGGGATAGTTGGGGGTGCTAGTATTTAGGGGCCAGAGGTGAAATTCTTGGATTCTCTAAAGACTAACTTATGCGAAAGCATTCACCAAGGATGTCTTCTTTAATCAAGAACGAAAGTTGGGGGATCGAAGACGATCAGATACCGTCGTAGTCTCAACCATAAACTATGCCAACTAGGGATGAGTGAACGTTGCATTTTCGACTTCATTCGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTTAGGATTGACAGGTTGAAGACCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCGACCTGCTAAATAGTTCAGCGTATCTTAGGATACGTCTGTGACTTCTTAGAGGGACTATCAGGAGAGTATCCGATGGAAGTTCGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATTCGTTCATCGAGCTCCCAACCTTGACCGGTAGGTCTGGGTAATCTTCTCAAAGCGAATCGTGCTGGGGATAGATTATTGTAATTTTTAATCTTCAACGAGGAATTCCTAGTAAACGCAAGTCATCAACTTGCATTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGCTCCATGGACTGT
Importance of organism and transcriptomes	Cercozoans are frequently found in 18S rRNA clone library surveys of marine environments and are thus considered ubiquitous in marine environments. They likely consume bacteria including cyanobacteria as prey, and thus are involved in recycling of nutrients through the microbial loop. Their biology is not well studied and we could not find a genome project for this group. This isolate was obtained from the SIO pier (UCSD, La Jolla, CA), has been in culture for more than a year and grows on a single Synechococcus strain and contaminating bacteria as food. This group would be important for phylogenetic reasons as part of a poorly sampled superfamily and for ecological reasons. We would hope to compare its transcriptome during growth with and without Synechococcus and to other nanoflagellates growing with and without Synechococcus.
Additional citations and references
Primary citation for organism's characterization, if available	Bass, D; Howe, AT; Mylnikov, AP et al. (2009) Phylogeny and Classification of Cercomonadida (Protozoa, Cercozoa): Cercomonas, Eocercomonas, Paracercomonas, and Cavernomonas gen. Nov. PROTIST Volume: 160 Issue:4 Pages:483-521
Experimental Data
Date of experiment	15-MAR-11
Growth medium	f/4 amended seawater
Temperature (ºC)	20
Salinty (psu)	34
pH	8
Light (µmol photons / m2 / sec)	20
Day portion of day:night cycle in hours	24
Nitrate (μmol/L)	441
Phosphate (μmol/L)	18
Trace elements (total) (nmol/L)	12.25
Prey organism, if applicable (genus and species)	Synechococcus CC9311