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Alexandrium monilatum CCMP3105


Principle Investigator(s) Sonya Dyhrman, Andrew Juhl
External sample IDAm, replete, modern CO2
Sample accession numberCAM_SMPL_002396
Assembly accession numberCAM_ASM_000214
Combined Assembly NameAlexandrium-monilatum-CCMP3105
Prelim. NCBI Taxon ID311494
18S rRNA
Importance of organism and transcriptomesWe are nominating Alexandrium monilatum as a representative toxic dinoflagellate among 6 marine phytoplankton species (from 4 Phyla) that together form a comparative study of how physio/chemical parameters, including future ocean scenarios, affect the physiological ecology of key bloom-forming microeukaryote species (please see additional nominations from PIs Dyhrman and Juhl). Each of the 6 species will be grown under identical physiological stresses or treatments (see below), allowing direct comparisons between major phytoplankton groups to the same suite of experimental treatments, and between treatments within each species, while maximizing transcriptome coverage over differentially regulated genes. A. monilatum is less well studied from a molecular perspective than some of the other Alexandrium species, and because of its basal position within the genus (Rogers et al. 2006), provides an important comparison to the more well-known members of the genus. A. monilatum is ecologically distinct from many other Alexandrium species because it prefers warm water (Juhl 2005) and does not produce saxitoxins, although it does produce other toxins that can cause massive fish kills and shellfish mortality (May et al. 2010). The strain JR08 was isolated from a dense bloom sample collected in Mississippi Sound USA in Sept 2000 (Juhl 2005). It forms long chains, swims vigorously, and is strongly bioluminescent. Bioluminescence and cell division in this species are tightly coupled to the day/night cycle suggesting dramatic differences in gene expression between day and night samples.
Additional citations and references
Environmental Data
Experimental Data
Date of experiment10-JAN-12
Growth mediumL1
Modifications to growth medium16N:1P
Temperature (ºC)24
Salinty (psu)32
Light (µmol photons / m2 / sec)100
Day portion of day:night cycle in hours14
Night portion of day:night cycle in hours10
Nitrate (μmol/L)576
Phosphate (μmol/L)36
Trace elements (total) (nmol/L)1000
CO2 (ppm)400
Pressure (atm)1
Investigation typeEukaryotes