Alexandrium monilatum CCMP3105

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Environmental Data
Principle Investigator(s)	Sonya Dyhrman, Andrew Juhl
External sample ID	Am, -N, modern CO2
NCGR Sample ID	MMETSP0095
Sample accession number	CAM_SMPL_002381
Assembly accession number	CAM_ASM_000199
Combined Assembly Name	Alexandrium-monilatum-CCMP3105
Genus	Alexandrium
Species	monilatum
Strain	CCMP3105
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	311494
18S rRNA	AGCCATGCATGTCTCAGTATAAGCATTAACCTGGCAAAACTGCAAATGGCTCATTAAATCAGTTATAATGCACTTGATAG TTGAATAATTACATGGATAACTGCGGTAATCCTATAGCTAATACATGCAATCAAACCTGACTCTTGTGGGAAGGTTGTGG TCATTAGTTTTCAGAACCAACCCAAACTCTGTTTGGAATATGCTGATTCACATTGACTGATGAATTACATGGCATTTGCC GGTAATGCATCATTCCAGTTTCTGACCTATCAGCTTCCGACGGTAAGGTATTGGCTTACCGTGGCAATGACGGGTAACGG AGGATTAGGGTTTGATTCCGGAGAGGGAGCTTGAGAAATGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACC CAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAAGGCATCCATGTTTTGTACTTGGAATGAATTGATTCTA AACTTTTCTATGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAA AATTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGTTGAGGGTGGCTGGTCCGCCCTCTGGGTGAGTATCTGGCACAA CCTTGGCATTCTCTTGAAAGCATAACTGCACTTGACTGTGTGGTGTGTTATCGAGTATTTTTACTTTGAGGAAATCAGAG TGTTTCAAGCAGGTGTTTGCCTTGAATACATTAGCATGGAATAATATTATAGGACCTTGGTTCTATTTTGTTGGTTTTTA AAGCTGAGGTGATGATAAATAGGGATAGTTGGGGGCATTCGTATTTAATTGTCAGAGGTGAAATTCTTGGATTTATTAAA GACGGACTACTGCGACAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGAT ACCGTCCTAGTCTTAACCATAAACCATGCCAACTAGAGGTTGAAAGTTGTTATCTATATGACTCCTTCAGTACCTTATGA GAAAATCAAAAGTCTTTGGGCTCCGGGGGGGAGTATGGTCGCAAGGGTGAAACTTAAAGGAATTGACGGAAGGGCACCAC CAGGAGTGGAGCCTGCGGCTTAATTTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAATGAGGATTGACAGATT GATAGCTTTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTT AACGAACGAGACCTTAACCTGCTAAATAGTTACACATAATTTCGGTTATGTGGGCAACTTCTTAGAGGGACTTTGTGTTT TTAACACAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATGT GTTCGATGAGTTTCAACCTTGCCTGGAAAGGCTGGGTAATCTTTTCAAAATGCATCGTGATGGGGTTTGATTATTGCAAT TATTAATCTTAAACGAGGAATTCCTAGTAAGCCTGAGTCATCAGCTTGTGCTGATTACGTCCCTGCCCTTTGTACACACC GCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTTGAACTGTAGCAATGTTCAGTTCTTGAGCAATGCTATGGA AAGTTTAATGAACCTTATCACTAGAGAAGAG
Importance of organism and transcriptomes	We are nominating Alexandrium monilatum as a representative toxic dinoflagellate among 6 marine phytoplankton species (from 4 Phyla) that together form a comparative study of how physio/chemical parameters, including future ocean scenarios, affect the physiological ecology of key bloom-forming microeukaryote species (please see additional nominations from PIs Dyhrman and Juhl). Each of the 6 species will be grown under identical physiological stresses or treatments (see below), allowing direct comparisons between major phytoplankton groups to the same suite of experimental treatments, and between treatments within each species, while maximizing transcriptome coverage over differentially regulated genes. A. monilatum is less well studied from a molecular perspective than some of the other Alexandrium species, and because of its basal position within the genus (Rogers et al. 2006), provides an important comparison to the more well-known members of the genus. A. monilatum is ecologically distinct from many other Alexandrium species because it prefers warm water (Juhl 2005) and does not produce saxitoxins, although it does produce other toxins that can cause massive fish kills and shellfish mortality (May et al. 2010). The strain JR08 was isolated from a dense bloom sample collected in Mississippi Sound USA in Sept 2000 (Juhl 2005). It forms long chains, swims vigorously, and is strongly bioluminescent. Bioluminescence and cell division in this species are tightly coupled to the day/night cycle suggesting dramatic differences in gene expression between day and night samples.
Additional citations and references
Experimental Data
Date of experiment	10-JAN-12
Growth medium	L1
Modifications to growth medium	Low N
Temperature (ºC)	24
Salinty (psu)	32
pH	8.3
Light (µmol photons / m2 / sec)	100
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	5
Phosphate (μmol/L)	36
Trace elements (total) (nmol/L)	1000
CO2 (ppm)	400
Pressure (atm)	1
Investigation type	Eukaryotes