Marine Microbial Eukaryote Transcriptome Sequencing Project
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Unknown Unknown NY0313808BC1
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| Principle Investigator(s) | Bassem Allam |
|---|---|
| External sample ID | QPX2 |
| NCGR Sample ID | MMETSP0099_2 |
| Sample accession number | CAM_SMPL_002391 |
| Assembly accession number | CAM_ASM_000209 |
| Combined Assembly Name | Undescribed-sp-isolateNY0313808BC1 |
| Genus | Unknown |
| Species | Unknown |
| Strain | NY0313808BC1 |
| Clonal | Yes |
| Axenic | Yes |
| Prelim. NCBI Taxon ID | 96639 |
| 18S rRNA | |
| Importance of organism and transcriptomes | The thraustochytrid known as QPX (Quahog Parasite Unknown) has caused significant disease and mortality in aquacultured and wild populations of the hard clam Mercenaria mercenaria along the east coast of North America since the 1950?s. Available knowledge on QPX is extremely limited. Generated new sequence data will allow the identification of factors involved in QPX virulence and pathogenicity offering a better understanding of conditions affecting the occurrence of disease outbreaks. |
| Additional citations and references | Perrigault M, Allam B. (2009). Cytotoxicity of Quahog Parasite Unknown (QPX) toward hard clam (Mercenaria mercenaria) haemocytes and interactions between different pathogen isolates and host strains. Parasitology 136: 1281-1289. Perrigault M, Bugge DM., Allam B. (2010). Effect of environmental factors on survival and growth of quahog parasite unknown (QPX) in vitro. Journal of Invertebrate Pathology 104: 83-89. |
| Environmental Data | |
| Primary citation for organism's characterization, if available | Qian H., Liu Q., Allam B., Collier J.L. (2007). Molecular genetic variation within and among isolates of QPX (Thraustochytridae), a parasite of the hard clam (Mercenaria mercenaria). Diseases of Aquatic Organisms 77: 159-168. |
| Latitude | 40.49657 |
| Longitude | -74.17591 |
| Depth (m) | 10 |
| Collection date | 01-NOV-03 |
| Collection time | 09:00 AM |
| Sample collection site | Atlantic_Ocean |
| Other collection site info | Raritan Bay, New York |
| Sample material (e.g. "seawater," "sediment," etc.) | Clam tissue |
| ENVO term for habitat - primary term | Acquatic: marine |
| ENVO term for habitat - secondary term | Not Applicable |
| Habitat | marine habitat |
| Country | UNITED STATES |
| Experimental Data | |
| Date of experiment | 01-APR-11 |
| Growth medium | Minimal Essential Medium with 10% fetal bovine serum |
| Temperature (ºC) | 13 |
| Salinty (psu) | 35 |
| pH | 7.2 |
| Investigation type | Eukaryotes |
| Other experimental metadata available | Isolate grown in 25 cm2 culture flasks in ambient air |