Acanthoeca-like sp. 10tr

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Environmental Data
Principle Investigator(s)	Brian Palenik
External sample ID	AcanthoecalikeNOSYN
NCGR Sample ID	MMETSP0105_2
Sample accession number	CAM_SMPL_002387
Assembly accession number	CAM_ASM_000205
Combined Assembly Name	Acanthoeca_like-sp-10tr
Genus	Acanthoeca-like
Species	sp.
Strain	10tr
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	81532
18S rRNA	TAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAATAGCTTGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTATTTGATAGTGTTTACTACATGGATACCCGTGGTAATTCTAGAGCTAATACATGCGAAAATGCCCGACTTCTGGGAGGGCTGTATTTATTAGATAAAAAACCAGCATTTTGTGTTGAATCATAATAACTTCGCGAATCGTTGAGCTTTATGCCGTCGATGAATCATTCAAATTTCTGCCCTATCAACTTTCGACGGTAGGGTATTGGCCTACCGTGGTTGCAACGGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATACGGAGCCTTTTAGGTTTTGTAATTGGAATGAGTACAATCTAAATCACTTAACGAGTAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGATGGATGGGATTGGTCCACCTCGCGGTGCGCACTGGTTCTGTCTGTTATTCTCTTGTTGGCGGATGGTGGCCTTAGTTGGTTGCTGTTTGTGCGCAAGACCTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGATACGCTTGAATACATTAGCATGGAATAATGGAATAGGACTTTGGTTCTATTTTGTTGGTTTCTAGGACCGAAGTAATGATTAATAGGGACAGTTGGGGGCATTAGTATTTAATTGTCAGAGGTGAAATTCTTGGATTTATGAAAGACTAACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTCAGGATCATCGAGGTGACAATAGCCACGGTGGCACTGTAGGAGAAATCAAAGTTTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACCAGGTCCAGACATAGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACACGATTCTCGTTAATCGTGGGCAACTTCTTAGAGGGACTATCGATGTTTAATCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGACAGGGACAACGAGTTTACACACCTTGCCCGGTAGGGCTGGGTAACCTTTTGAGACCTTGTCGTGTTGGGGCTAGATCTTTGTAATTTTCGATCTCAAACGAGGAATTCCTAGTAAGCGTGATTCATCAGATCGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGTGAGATCTCGGGACTGGCGCGATGGCGCTGGCAACAGCATTGTCGTGCCGGGAACCTGCTCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAG
Importance of organism and transcriptomes	Heterotrophic nanoflagellates consume bacteria including cyanobacteria as prey, and thus are involved in recycling of nutrients through the microbial loop. This isolate resembles Acanthoeca spectabilis Ellis 1930, but its 18S rRNA sequence is only 94% identical and it is closest to uncultured environmental clones from a Norwegian fjord. Speciation of these organisms in the marine environment is clearly poorly understood. the culture was obtained from the SIO pier (UCSD, La jolla, CA), has been in culture for more than a year and grows on a single Synechococcus strain and contaminating bacteria as food. It also grows on the small alga Ostreococcus choanoflagellates are poorly studied in marine environments, but a genome of a choanoflagellate Monosiga brevicollis has been determined which provides a rich resource for compairsions to this isolate. we would hope to compare the Acanthoeca-like transcriptome during growth with and without synechococcus and to other nanoflagellates growing with and without Synechococcus.
Additional citations and references	Behnke,A., Barger,K.J., Bunge,J. and Stoeck,T. (2010) Spatio-temporal variations in protistan communities along an O/HS gradient in the anoxic Framvaren Fjord (Norway) FEMS Microbiol. Ecol. 72 (1), 89-102.
Primary citation for organism's characterization, if available	Carr,M., Leadbeater,B.S., Hassan,R., Nelson,M. and Baldauf,S.L. (2008) Molecular phylogeny of choanoflagellates, the sister group to Metazoa Proc. Natl. Acad. Sci. U.S.A. 105 (43), 16641-16646
Experimental Data
Date of experiment	15-MAR-11
Growth medium	f/4 amended seawater
Temperature (ºC)	20
Salinty (psu)	34
pH	8
Light (µmol photons / m2 / sec)	20
Day portion of day:night cycle in hours	24
Nitrate (μmol/L)	441.23
Phosphate (μmol/L)	18.15
Trace elements (total) (nmol/L)	12.25
Prey organism, if applicable (genus and species)	Unknown bacteria