Goniomonas Pacifica CCMP1869

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Environmental Data
Principle Investigator(s)	Brian Palenik
External sample ID	GoniomonasNOSYN
NCGR Sample ID	MMETSP0107_2
Sample accession number	CAM_SMPL_002385
Assembly accession number	CAM_ASM_000203
Combined Assembly Name	Goniomonas-pacifica-CCMP1869
Genus	Goniomonas
Species	Pacifica
Strain	CCMP1869
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	195067
18S rRNA	TAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTGTAAATAAGTCTACACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATGGTCGCTTACTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCATCAAGCCCCAACTTCGGAAGGGGTGTATTTATTAGATTCAAAACCAACCTTGGTGATTCATAATAACTTCTCGAACCGCATGGCCTTGTGCTGGCGGTGATTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATATAGGCCTACCATGGTTTTAACGGGTGACGGAGAATTAGGGTTCGGTTCCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACGCGGGGAGGTAGTGACAATAAATAACAATACAGGGCTCACAGAGTCTTGTAATTGGAATGAGAACAATTTAAATCTCTTAACGAGGATCAATTAGAGGGCAAGTCTGGTGCCTGCAGCCGCGGTAATTCCAGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTATTGGATCTCGGGGTCGGAACGGCTGTCGGGCGATGCTCGGACGGCCGGTTTGGCTCCTTTCTTCCGAGGCTTGCGTCTCTTTCACTAGAGGGCGTTGGGGCTCGGATCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGCCCTTCCTTGAATACTTACATGGAATAATGGAATACGACCGCCGTTCTATTTTGTTGGTTTCAGGACCGAAGTATTGATTAAAAGGATCGTTGGGGCCGTTTGTTTCCGTTGTCAGAGGTGAAATTCTTGGATTTACCGAAGACAAACTTCTGCGAAAGCCTCGGCCAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCAGTGGACGTTGTTTTACGACTCCATTGGCACCTTGTGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATCGACGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTGAAAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCGCAGCCTGCTAAATGTCACGCCAACAATTGCGTTGGCGGCCCTTCTTAGAGGGACTATTTGTGTTTAACGAATGGAAGTTTGCGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGAATTCAACGAGCTCACAACCTTGACCGAAAGGCCCGGGTAAACTCCGAAATTTCATCGTGATGGGGATAGACTATTGTAATTATTAGTCTTCAACGAGGAATTCCTAGTAAGCGCGATTCATCAGATCGCGTTGATTACGTCCCTGCCCTTTGAACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGATTTCGGATTGCTGGCCTTGCCTTGGCAACAGGGCTTGGTTGCAAAAAGTTGATCAAACTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCC
Importance of organism and transcriptomes	Goniomonas species are related to cryptophytes, but have lost a functioning chloroplast. They consume bacteria including cyanobacteria and small phytoplankton as prey, and thus are involved in recycling of nutrients through the microbial loop. They have been important model organisms for studying grazer-prey dynamics. Their biology is not well studied and we could not find a genome project for this group. Recently a related group of colorless cryptophytes has been determined to be widespread. This goniomonas isolate was obatined from the CCMP and has been used in grazing experiments with different strains in our labs. We would hope to compare its transcriptome during growth with and without Synechococcus and to other nanoflagellates growing with and without Synechococcus
Additional citations and references	Martin-Cereceda M, Roberts EC, Wootton EC, Bonaccorso E, Dyal P, Guinea A, Rogers D, Wright CJ, Novarino G. (2010) Morphology, ultrastructure, and small subunit rDNA phylogeny of the marine heterotrophic flagellate Goniomonas aff. amphinema. J Eukaryot Microbiol 57(2):159-70.
Primary citation for organism's characterization, if available	Deane,J.A., Strachan,I.M., Saunders,G.W., Hill,D.R.A. and McFadden,G.I. (2002) Cryptomonad evolution: nuclear 18S rDNA phylogeny versus cell morphology and pigmentation J. Phycol. 38 (6), 1236-1244.
Experimental Data
Date of experiment	15-MAR-11
Growth medium	f/4 amended seawater
Temperature (ºC)	20
Salinty (psu)	34
pH	8
Light (µmol photons / m2 / sec)	20
Day portion of day:night cycle in hours	24
Nitrate (μmol/L)	441.23
Phosphate (μmol/L)	18.15
Trace elements (total) (nmol/L)	12.25
Prey organism, if applicable (genus and species)	Unknown bacteria