Bicosoecid sp ms1

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Environmental Data
Principle Investigator(s)	Eunsoo Kim
External sample ID	Bicoso
NCGR Sample ID	MMETSP0115
Sample accession number	CAM_SMPL_002409
Assembly accession number	CAM_ASM_000227
Combined Assembly Name	N/A
Genus	Bicosoecid
Species	sp
Strain	ms1
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	33651
18S rRNA	GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAACGCTTTTATACTGTGAAACTGCGAATGGCTCATTATATC AGTTATAGTTTATTTGATAGTCCCCTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGTAAAGCG CCGACTCTTGTGGGAAGGCGTGTATTTATTAGATATAAACCAATGCGGGTTCTGCCCGGTATTTGGTGAGTCATA ATAACTGAGCGAATCGCAGTGGCCTTCGGGTCGGCGATGGTTCATTCAAGTTTCTGCCCTATCAGCTTTCGATGG TAGGGTATTGGCCTACCATGGCATTAACGGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAGA CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTAACTCAGGGAGGTAGTGACAATAAAT AACAATGCGGGGCTTCTATAGTCTTGCAATTGGAATGAGTAGAATGTAAAATCCTTTACGAGGATCAATTGGAGG GCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTC GTAGTTGGATTTCTGGATGGAGCGACCGGTCCGCGTCCTCTGGGCGTGTGTACTTGAGCGCCGCATCCATTCTCG CGGGGAACCGTCTGGCGCTCACGCGTCGGGCGAGGATCCGCGTCGTTTACTGTGAAAAAATTAGAGTGTTTAAAG CAGGCTTATGCCTTGAATACATTAGCATGGAATAATAAGATAGGACTTTGGCCTATTTTGTTGGTTTGCAAGCCG AGGTAATGATTAATAGGGATAGTTGGGGGTATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTATGAAAG ACGAACAACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTCAGGGGATCGAAGAGGATT AGATACCCTCGTAGTCTTGACCATAACCTATGCCGACTCGGGATTGGTGGAAGTTCTCTTATGACTCCATCAGCA CCGTATGAAAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGA AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCGGACATAGTG AGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGAT CTGTCTGGTTAATTTCGACAACGAACGAGACCTCCGCCTGCTAAATAGCAGCGCGATGCATTGCATCGTGGCAGC TTCTTAGAGGGACTTTGGGTGTTTAGCCCAAGGAAGTTGGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTC TGGGCCGCACGCGCACTACACTGACTGGTGCAACAAGTTTATAACCTTGGTCGATGGGCCTGGGTAATCTTTGAA CGCCAGTCGTGATGGGGATAGATTCTTGCAATTATTAATCTTCAACGAGGAATTCCTAGTAAACGCAAGTCATCA GCTTGCATTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAACC TCTGGACCGAGGCGATCTGCGCAAGTGGATCGTTCACGGGAAGTTGGTTAAACCTTACCATTTAGAGGAAGGTGA AGTCGTAACA
Importance of organism and transcriptomes	The strain was isolated from a sandy beach in Prince Edward Island, Canada and reproduces by vegetative cell division. Its 18S rDNA sequence suggests that it is a novel, as yet unnamed, protist that branches deeply within the Bicosoecida, a group of free-living, bactivorous flagellates (e.g., Cafeteria) that commonly occur in various aquatic habitats and play important roles in the food web. In addition to improve our understanding of the eukaryotic tree of life, comparing the transcriptomes of heterotrophic stramenopiles to those of plastid-bearing stramenopiles will provide key insight into the genomic changes accompanying the transition from obligate heterotrophy to phototrophy.
Additional citations and references
Latitude	46.5105
Longitude	-63.4856
Temperature (ºC)	20
Collection date	01-JUL-10
Collection time	11:00 AM
Sample collection site	Atlantic_Ocean
Sample material (e.g. "seawater," "sediment," etc.)	seawater
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Not Applicable
Habitat description	Sandy beach
Habitat	marine habitat
Country	CANADA
Experimental Data
Date of experiment	10-MAR-11
Growth medium	ESM plus SW802
Modifications to growth medium	No modification
Temperature (ºC)	23
Salinty (psu)	35
pH	7.5
Night portion of day:night cycle in hours	24
Nitrate (μmol/L)	705
Phosphate (μmol/L)	14
Trace elements (total) (nmol/L)	68
Investigation type	Eukaryotes