Symbiodinium kawagutii CCMP2468

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Environmental Data
Principle Investigator(s)	Senjie Lin
External sample ID	SymkaSL1
NCGR Sample ID	MMETSP0132_2C
Sample accession number	CAM_SMPL_002423
Assembly accession number	CAM_ASM_000241
Combined Assembly Name	Symbiodinium-kawagutii-CCMP2468
Genus	Symbiodinium
Species	kawagutii
Strain	CCMP2468
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	104179
18S rRNA	gcttgtctcaaagattaagccatgcATGTCTCAGTATAAGCATTCAAACGGCGAAACTGCGAATGGCTCATTAAAGCAGTTATAGTTTATTTGATGGTCGCTGCTACATGGATAACTGTGGTAATTCTAGAGCTAATACATGCATCCAAGCCCGACTTCGCAGAAGGGTTGTGTTTATTAGATACAGAACCAACCCAGGTTCTACCTGGTCATGTGGTGATTCATGATAACTTGACGAATCGTGTGGCCTTGCCGACGATGCGTCATTCAAGTTTCTGACCTATCAGCTTCCGACGGTAGGGTATTGGCCTACCGTGGCAATGACGGGTAACGGAGAATTAGGGTTTGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGAATGAGTAGAACTTAAATCTCTTTATGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGTTGAGGATGACCGGTCCGCCCTCTGGGTGTGTATCTGGCTCAGCCTTGACATCTTCCTGAAGAACGTATCTGCACTTGACTGTGTGGTGCGGTATTTGGGACATTTACCTTGAGGAAATTAGAGTGTTTCAAGCAAGCGCGCGCTTTGAATACATTAGCATGGAATAATAAGATAGGACCTCAGTTCTATTTTGTTGGTTTCTAGAGCTGAGGTAATGATTGATAGGGATAGTTGGGGGCATTCGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACGGACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCCTAGTCTTAACCATAAACCATGCCAACTAGAGATTGGAGGTCGTTACTTGAATGACTCTTTCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACATGTAACCTCGGTTACATGGGCAACTTCTTAGAGGGACTTTGTGTGTCTAACGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCGCTCAACGAGTTTATGATCTTGCCTGAAATGGTTGGGTAATCTTTTTAAAATGCATCGTGATGGGGATAGATCATTGCAATTATTGATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGTGCTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTTGGACCGACGCAGTGCTCAGCTTCTGGACGTTGCGTTGGAAAGTTTCATGAACCTTATCACTTAGAGGAAGGAGAAgtcgtaacaaggtttccgtaggtg
Importance of organism and transcriptomes	Symbiobiodinium spp. are essential endosymbionts of reef building coral. Current widespread coral bleaching is due to expelleing of this endosymbiont as a consequence of environmental stress. S. kawagutii was originated from coral reef near Hawaii, and has not been systematically studied with respect to gene expression. RNA samples prepared from cultures grown under stress and various normal conditions as planned will lead to a transcriptome dataset valuable for understanding how environmental stress leads to expelling of this alga from the corals.
Additional citations and references
Primary citation for organism's characterization, if available	Zhang, Bhattacharya and Lin 2007. J. Mol. Evol. 65: 463-474
Latitude	21.25
Longitude	158
Salinity (psu)	35
Sample collection site	Pacific_Ocean
Other collection site info	coral reef near Hawaii
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	15-APR-11
Growth medium	L1 amended seawater
Temperature (ºC)	30
Salinty (psu)	31
pH	8.1
Light (µmol photons / m2 / sec)	200
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36.2
Trace elements (total) (nmol/L)	11.7
Investigation type	Eukaryotes
Other experimental metadata available	submitted are total RNA