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Pseudo-nitzschia australis 10249 10 AB


Principle Investigator(s) G Jason Smith
External sample ID10249 10AB_NH4
NCGR Sample IDMMETSP0140_2
Sample accession numberCAM_SMPL_002418
Assembly accession numberCAM_ASM_000236
Combined Assembly NamePseudo_nitzschia-australis-10249_10_AB
Strain10249 10 AB
Prelim. NCBI Taxon ID44445
18S rRNA
Importance of organism and transcriptomesPseudo-nitzschia australis is a ubiquitous toxigenic diatom capable of producing the potent neurotoxin domoic acid (DA). This study seeks to compare the transcriptomes of P. australis when maintained at equivalent growth rates utilizing different N-sources (NO3 vs NH4, UREA, GLUTAMATE) in order to gain an understanding of the physiological scope of this species to utilize reduced nitrogen sources to support growth, DA biosynthesis and general amino acid metabolism.
Additional citations and references
Environmental Data
Depth (m)5
Salinity (psu)33
Temperature (ºC)15
Collection date06-SEP-10
Collection time10:00 AM
Sample collection sitePacific_Ocean
Other collection site infoMonterey Commercial Wharf II, part of routine time series sampling for Pseudo-nitzschia
Sample material (e.g. "seawater," "sediment," etc.)Seawater
Nirate (μmol/kg)4
Phosphate (μmol/kg)0.2
Silicate (μmol/kg)6.1
ENVO term for habitat - primary termAcquatic: marine
ENVO term for habitat - secondary termNot Applicable
Habitat descriptioncoastal ocean bay, near small boat harbor
Habitatmarine habitat
Experimental Data
Date of experiment04-AUG-11
Growth medium0.2u filtered Monterey Bay seawater with ES enrichments lacking N- source which is applied in experiment specific manner
Modifications to growth mediumContinuous culture with dilution rate set to 0.29/d, NH4 as N source
Temperature (ºC)15
Salinty (psu)33
Light (µmol photons / m2 / sec)250
Day portion of day:night cycle in hours24
Ammonium (μmol/L)60
Phosphate (μmol/L)36.7
Silicate (μmol/L)105.6
Total Fe (nmol/L)6560
Trace elements (total) (nmol/L)2687
Pressure (atm)1
Investigation typeEukaryotes
Other experimental metadata availableContinuous culture with dilution rate set to 0.29/d