Pseudo-nitzschia australis 10249 10 AB

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Environmental Data
Principle Investigator(s)	G Jason Smith
External sample ID	10249 10AB_GLUTAMATE
NCGR Sample ID	MMETSP0142_2
Sample accession number	CAM_SMPL_002415
Assembly accession number	CAM_ASM_000233
Combined Assembly Name	Pseudo_nitzschia-australis-10249_10_AB
Genus	Pseudo-nitzschia
Species	australis
Strain	10249 10 AB
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	44445
18S rRNA	TGATCCTGCCAGTAGTCATACGCTCGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAATATTTTACTTTGAAACTGC GAACGGCTCATTATATCAGTTATAGTTTATTTGATAGTCCCTTACTACTTGGATACCCGTAGTAATTCTAGAGCTAATAC ATGCGTCAATTCCCTTCTGGGGAAGTATTTATTAGATTGAAACCAACCCCTTCGGGGTGATGTGGTGATTCATAATAAGC TTGCGGATCGCATGCCTCTGGCGGCGATGGATCATTCAAGTTTCTGCCCTATCAGCTTTGGATGGTAGGGTATTGGCCTA CCATGGCTTTAACGGGTAACGGGAAATTAGGGTTTGGATTCCGGAGAGGGAGCCTGAGAGACGGCTACCACTATTCAAGG AAGGGCAGCAGGCGCGTAAATTACCCAATCCTGACACAAGGAGGTAGTGACAATAAATACAAATGCCGGGCCTTCTTAGG TCTGGCAATTGGAATGAGAACAATTTAAACCCCTTATCGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT AATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTGTGGTGTGTCCAGTCGGCC TTTGCTCTTTGAGTGATTGCGCTGTACTGGTCTGCCATGTTTGGGTGGAATCTGTGTGGCATTAAGTTGTCGTGCAGGGG ATGCCCATCGTTTACTGTGAAAAAATTAGAGTGTTCAAAGCAGGCTTATGCCGTTGAATATATTAGCATGGAATAATGAT ATAGGACCTTGGTACTATTTTGTTGGTTTGCGCACTAAGGTAATGATTAAGAGGGACAGTTGGGGGTATTTGTATTCCAT TGTCAGAGGTGAAATTCTTGGATTTTTGGAAGACAAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGA ACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACAAGGGATTGGCGGAAC GACGTTACGTCTCCGTCAGCACCTTGTGAGAAATCATAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAAC TTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGT CCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG GAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCCCTGCCTGCTAAATAGCACGCATAATGTTTATCATTGGTGT AATGCTTCTTTAAAGGGACGTGGCCGTTCTATTAAACCCAGGAAGATAAGGGGCAATTAACAGGTCCTGTGGATGCCCTT AGATGTTTCTGGGCCGCACTCCGCGCCTAACACTGTGATGTATTCACTAGTCTACTCTTGGCCGAAAGGCCTGGGCAATC TTTTGAACTTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAAACGCAGATCAT CAATCTGCATTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGCCTCG GGATTGTGGTTAGTTTCCTTTATTGGAAGGTAGTCACGAGAACCTGTCTAAACCTTATCATTTAGAGGAAGGTGAAGTCG TAACAAGGTTTCCGTAGGTGAACGCCAAAG
Importance of organism and transcriptomes	Pseudo-nitzschia australis is a ubiquitous toxigenic diatom capable of producing the potent neurotoxin domoic acid (DA). This study seeks to compare the transcriptomes of P. australis when maintained at equivalent growth rates utilizing different N-sources (NO3 vs NH4, UREA, GLUTAMATE) in order to gain an understanding of the physiological scope of this species to utilize reduced nitrogen sources to support growth, DA biosynthesis and general amino acid metabolism.
Additional citations and references
Latitude	36.60369
Longitude	-121.88927
Depth (m)	5
Salinity (psu)	33
Temperature (ºC)	15
Collection date	06-SEP-10
Collection time	10:00 AM
Sample collection site	Pacific_Ocean
Other collection site info	Monterey Commercial Wharf II, part of routine time series sampling for Pseudo-nitzschia
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
Nirate (μmol/kg)	4
Phosphate (μmol/kg)	0.2
Silicate (μmol/kg)	6.1
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Not Applicable
Habitat description	coastal ocean bay, near small boat harbor
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	04-AUG-11
Growth medium	0.2u filtered Monterey Bay seawater with ES enrichments lacking N- source which is applied in experiment specific manner
Modifications to growth medium	Continuous culture with dilution rate set to 0.29/d, GLUTAMATE as N source
Temperature (ºC)	15
Salinty (psu)	33
pH	8.2
Light (µmol photons / m2 / sec)	250
Day portion of day:night cycle in hours	24
Phosphate (μmol/L)	36.7
Silicate (μmol/L)	105.6
Total Fe (nmol/L)	6560
List of amino acids and concentrations, with units	Glutamate, 80 umole/L
Trace elements (total) (nmol/L)	2687
Pressure (atm)	1
Investigation type	Eukaryotes
Other experimental metadata available	Continuous culture with dilution rate set to 0.29/d