Chaetoceros debilis MM31A-1

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Environmental Data
Principle Investigator(s)	Bank Beszteri
External sample ID	C. debilis CTR 2
NCGR Sample ID	MMETSP0149
Sample accession number	CAM_SMPL_002437
Assembly accession number	CAM_ASM_000255
Combined Assembly Name	Chaetoceros-debilis-MM31A_1
Genus	Chaetoceros
Species	debilis
Strain	MM31A-1
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	122233
18S rRNA	aacctgggtttggtcctgccagtagtcatacgctcgtctcaaagattaagccatgcatgtgtaagtataaatattatatcttaaaactgcgaacggctcattatatcagttgtaatctctttcgtaatcccttactacttggataaccgtagtaattctagagctaatacatgcataaatatcacgtagtgatagtgcttattagattgaaaccaacttccttcgggaagattttggtgactcacaataactttgcgaatcgcatgctttttgcggcgatggacgaatcgagcttctgccctatcagctttgatggctgtgtattggacagccatggctttaacgggtaacggagaattagggtttgattccggagagggagcctgagaaacggctaccacatctaaggaaggcagcaggcgcgaaaattacccaatactaatacagtgaggtagtaacaataaataacacagcccagcatttcatgttgggcttttggaatgagaacaatttaaatccattatcgaggatcaattggagggcaagtctggtgccagcagccgcggtaattccagctccaatagcgtatattaaagttgctgcagttaaaaagctcgtagttggatttctagcgaggcacgagaatatccacctatggtgaatacttctctcgccttgctcttctttagttgatgtctctggcattaagttgtcggagtcctgagactaatcgtttactgtgagaaaattagagtgttcaaagcaggcttatgccgttgaatatattagcatggaataataagataggacctcggtactattttgttggtttgcgtactgatgtaatgataaatagggactggcgtgggtattcgtattatgatgtcagaggtgaaattcttagattatcgtaagacgaacgactgcgaaagcatttaccaaggatgttttcattaatcaagaacgaaagtatggggatcgaagatgattagataccatcgtagtctataccataaactatgccgactcagggtggtctgtcgatttaacgatagactgaactgtaggagaaatcaaagtctttgggttccggggggagtatggtcgcaaggctgaaacttaaagaaattgacggaagggcaccaccaggagtggaacctgcggcttaatttgactcaacacgggaaaacttatcaggtccagacaaagtgaggattgacagattgagagctctttcttgattctttgggaggtggtgcatggccgttcttagttggtggagtgatttgtctggtttattccgttaacgaacgagaccctcgcctgctaaataatcgattttatcaatttgatagattacggtttcttagagggacatgtagcattaagctacaggaagatgagggcgataacaggtctgtgatgcccttagatgttctgaaccgcacgcgtgttacaatggcacattcaacgagcttatccttgcccgagaggtctgggaaatcttctaaagtgtgccttgaaagggatcgatagctgcaattgttatcgtgaacgaggaattcctagtaaacgcatttcatcaaaatgcattgattacgtccctgccctttgtacacaccgcccgtcgcacctaccgattgaaaagcccggtgaagaatcgagatcgtagcgttgtcctttattggacattgccgtgagaacctttctgaaccttgttttttagaggaaggtgaagtcgtaacaaggtctccgtaggtgaacctcaaaaaagatcaa
Importance of organism and transcriptomes	C. debilis can attain extraordinary growth rates under favourable growth conditions and exhibits a "boom-and-bust" life cycle by rapidly increasing cell numbers by orders of magnitude and an abrupt termination of the bloom followed mass sedimentation which is accompanied by the formation of resting spores that serve as a seed bank for the initiation of future blooms. This sample is part of a comparative analysis of transcriptomic responses to iron limitation in a number of characteristic diatom species of the Southern Ocean.
Additional citations and references
Primary citation for organism's characterization, if available	Cleve PT (1894). Bih. Kongl. Svenska Vetensk.-Akad. Handl. 20(3/2):1-16
Latitude	-49.6
Longitude	2.1
Collection date	27-FEB-04
Sample collection site	Southern_Ocean
Other collection site info	EIFEX station 513
Sample material (e.g. "seawater," "sediment," etc.)	seawater
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Other
Other environmental metadata available	http://www.pangaea.de/search?q=eifex
Other environmental metadata available	http://www.pangaea.de/search?q=eifex
Habitat	marine habitat
Experimental Data
Date of experiment	17-MAY-11
Growth medium	modified f/2 amended seawater
Modifications to growth medium	Using trace metal solution after Morel et al. 1979. medium set up using trace metal clean conditions and procedures.
Temperature (ºC)	3
Salinty (psu)	33.6
pH	8.15
Light (µmol photons / m2 / sec)	35
Day portion of day:night cycle in hours	16
Night portion of day:night cycle in hours	8
Nitrate (μmol/L)	28
Ammonium (μmol/L)	.09
Phosphate (μmol/L)	28.45
Silicate (μmol/L)	60
Total Fe (nmol/L)	11000
Trace elements (total) (nmol/L)	7.3
Investigation type	Eukaryotes