Thalassionema nitzschioides L26-B

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Environmental Data
Principle Investigator(s)	Bank Beszteri
External sample ID	T. nitzschioides Fe- 1
NCGR Sample ID	MMETSP0158
Sample accession number	CAM_SMPL_002429
Assembly accession number	CAM_ASM_000247
Combined Assembly Name	Thalassionema-nitzschioides-L26_B
Genus	Thalassionema
Species	nitzschioides
Strain	L26-B
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	33649
18S rRNA	taattaacctggtttgatcctgccagtagtcatacgctcgtctcaaagattaagccatgcatgtctaagtataaatctcttactttgaaactgcgaacggctcattatatcagttataatttatttgatagtcccttactacttggataaccgtagtaattctagagctaatacatgcgtcaacacctttgggtggtatttattagaaagaaaccaactccttcgggatgatgtggtgaatcataataagtttgcgaatcgcacggctttgtgctggcgatggatcattcaagtttctgccctatcagctttggatggtagggtattggcctaccatggctttaacgggtaacgggaaattagggtttgattccggagagggagcctgagagacggctaccacatccaaggaaggcagcaggcgcgtaaattacccaatcctgacacagggaggtagtgacaataaataacaatgccgggcctttgtaggtctggcaattggaatgagaacaatttaaaccccttatcgaggaccaattggagggcaagtctggtgccagcagccgcggtaattccagctccaatagcgtatattaaagttgttgcagttaaaaagctcgtagttggatttgtggatttcgcggttcggtcgcactaattgtgtgtacatgagtcgtgagtccatccttgggtggaatctgtgtggcattaggttgtcgtgcaggtgatgcccatcgtttactgtgaaaaaattagagtgttcaaagcaggcttatgccgttgaatatattagcatggaataataagataggaccttggtactattttgttggtttgcgcaccgaggtaatgattaatagggacagttgggggtattcgtattccattgtcagaggtgaaattcttggatttttggaagacgaactactgcgaaagcatttaccaaggatgttttcattaatcaagaacgaaagttaggggatcgaagatgattagataccatcgtagtcttaaccataaactatgccgacaagggattggcggtcgttgtattgaccccgtcagcaccttatgagaaatcacaagtctttgggttccggggggagtatggtcgcaaggctgaaacttaaagaaattgacggaagggcaccaccaggagtggagcctgcggcttaatttgactcaacacgggaaaacttaccaggtccagacatagtgaggattgacagattgagagctctttcttgattctatgggtggtggtgcatggccgttcttagttggtggagtgatttgtctggttaattccgttaacgaacgagacccctgcctgctaaatagttttgctaatgatttttcattggtatcgtaacttcttagagggacgtgcgttctattagacgcaggaagataggggcaataacaggtctgtgatgcccttagatgtcctgggccgcacgcgcgctacactgatgcatgcaacgagtgtaccttggccgagaggcctgggaaatctgtgaacgtgcatcgtgatagggatagattattgcaattattaatcttgaacgaggaattcctagtaaacgcagatcatcagtctgcattgattacgtccctgccctttgtacacaccgcccgtcgcacctaccgattgaatggtccggtgaagcctcgggattgtgatgagtttcctttattggggatttgtcgcgagaacttgtctaaaccttatcattagaggaaggtgaagtcacatccacgtttccgtacgtgaacctgcaaaaaagat
Importance of organism and transcriptomes	T. nitzschioides is a characteristic diatom species of phytoplankton communities of the Antarctic Circumpolar Current and its frustules are also abundant in the siliceous ooze belt. This transcriptome is part of a comparative study of the transcriptomic responses of Southern Ocean diatoms to iron limitation.
Additional citations and references
Primary citation for organism's characterization, if available	(Grunow) Mereschkowsky 1902: 78
Latitude	-47.86667
Longitude	-15.8
Collection date	28-JAN-09
Sample collection site	Southern_Ocean
Other collection site info	LOHAFEX station 120
Sample material (e.g. "seawater," "sediment," etc.)	Calanus simillimus faecal pellet
ENVO term for habitat - primary term	Acquatic: marine
Other environmental metadata available	further metadata available at http://www.pangaea.de
Other environmental metadata available	further metadata available at http://www.pangaea.de
Habitat	marine habitat
Experimental Data
Growth medium	modified f/2 amended seawater
Modifications to growth medium	Using trace metal solution after Morel et al. 1979. medium set up using trace metal clean conditions and procedures.
Temperature (ºC)	3
Salinty (psu)	33.6
pH	8.15
Light (µmol photons / m2 / sec)	35
Day portion of day:night cycle in hours	16
Night portion of day:night cycle in hours	8
Nitrate (μmol/L)	28
Ammonium (μmol/L)	.09
Phosphate (μmol/L)	28.45
Silicate (μmol/L)	60
Trace elements (total) (nmol/L)	7.3
Investigation type	Eukaryotes