Odontella Sinensis Grunow 1884

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Environmental Data
Principle Investigator(s)	Declan Schroeder
External sample ID	Ply 606
NCGR Sample ID	MMETSP0160_2
Sample accession number	CAM_SMPL_002432
Assembly accession number	CAM_ASM_000250
Combined Assembly Name	N/A
Genus	Odontella
Species	Sinensis
Strain	Grunow 1884
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	2839
18S rRNA	AACCTGGTTGATCCTGCCAGTAGTCATACGCTCGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAATATTATACATTGAAACTGCGAATGGCTCATTATATCAGTTATAGTTTATTTGATAGTCCTTTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCATCAACACCCAACCGCTTGCGGACGGGTTGTATTTATTAGATAGAAACCAACCCTCTTCGGAGGTGCTTTGGTGATTCATAATAACTTTGCGAATCGCATGGCTCCACGCCGGCGATGGATCATTCAAGTTTCTGCCCTATCAGCTTTGGATGGTAGGGTATTGGCCTACCATGGCTTTAACGGGTAACGGATAATTAGGGTTAGATTCCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATGCCGGGCCTTTCTAGGTCTGGCAATTGGAATGAGAACAATTTAAATCCCTTATCGAGGATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTCTGGTGGGAGCAATCGGTCCGACGCTCTGCGTGGGTACTTGTATTGTCTCCCGCCATCCTTGGGGAGAGCCTGTCTGGCATTAGGTTGTCGGGCAGGGGATCCCCATCGTTTACTGTGAAAAAATTAGAGTGTTCAAAGCAGGCTTACGCCGTTGAATATATTAGCATGGAATAATAAGATAGGACCTCGGGACTATTTTGTTGGTTTGCGCCCCGTGGTAATGATTAATAGGGACAGTTGGGGGTATTCGTATTGTGCTGTCAGAGGTGAAATTCTTGGATTAGCGCAAGACGAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGATCGAAGATGATTAGATACCATCGTAGTCTCAACCATAAACTATGCCGACTCGGGATTGGCGGTCGTTACTAATGACTCCGTCAGCACCGTAAGGGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCCCTGCCTGCTAAATAGTCCAGTCAATGGTTATTCATTGGCGTGGGCTTCTTAGAGGGACGTCCATCTACTAGGTGGAGGAAGATAGGGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCCGCACGCGCGCTACACTGATGCACTCAACGAGCATATAACCTTGGCCGAGAGGCCTGGGCAATCTTGTTAACTTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAAACGCAGGTCATCAACCTGCATTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCTGGTAGCTTTATTGGTACCTGGCCGTGAGAACTTGTCCAAACCTTATCATTTAGAGGAAGGTGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATCAA
Importance of organism and transcriptomes	Due to its relative large size (apical axis: 240-320 ?m; width: 28-32 ?m), O. sinensis represents an excellent model species for cellular studies of silicification and physiological processes in the diatoms. We have made the remarkable discovery that O. sinensis cells exhibit plasma membrane action potentials resembling the biophysical and pharmacological characteristics of the action potential found in excitable animal cells (Taylor 2009). Moreover, recent evidence indicates these action potentials may act via a novel mechanism to mediate pH homeostasis in O. sinensis and may be impacted by ocean acidification.Deep sequencing of O. sinensis transcriptome will not only contribute to better understand the complex diatoms phylogeny and evolutionary history but will also bring for the first time the sequence information necessary to complement the molecular and physiological studies currently undertaken on this model diatom species.
Additional citations and references	Taylor AR A fast Na+/Ca2+-based action potential in a marine diatom. PLoS One. 2009;4(3):e4966.; Kowallik K, Stoebe B, Schaffran I, Kroth-Pancic P, Freier U. The chloroplast genome of a chlorophyll a+c- containing alga, Odontella sinensis. Plant Mol Biol Rep. 1995;13:336?342. doi: 10.1007/BF02669188.
Experimental Data
Date of experiment	01-APR-11
Growth medium	f/2
Temperature (ºC)	15
Salinty (psu)	36
pH	8.3
Light (µmol photons / m2 / sec)	150
Day portion of day:night cycle in hours	16
Night portion of day:night cycle in hours	8
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36
Silicate (μmol/L)	106
Trace elements (total) (nmol/L)	12800