Neoparamoeba aestuarina SoJaBio B1-5/56/2

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Environmental Data
Principle Investigator(s)	Jan Pawlowski
External sample ID	Neoparamoeba aestuarina SoJaBio B1-5/56/2
NCGR Sample ID	MMETSP0161_2
Sample accession number	CAM_SMPL_002433
Assembly accession number	CAM_ASM_000251
Combined Assembly Name	N/A
Genus	Neoparamoeba
Species	aestuarina
Strain	SoJaBio B1-5/56/2
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	180227
18S rRNA	CTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTTAAAGATTAAGCCATGCATGTCTAAGTATAAACACTTTGTACTTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAATTTATTTGATGGTCTTTTACTACTTGGATAACCGTGGTAAATCTAGAGCTAATACATGCACAAAATCCTGACTGCTTTCGGGTAGGAGGGATGTATTTATTAGATTCAAAAGCCAATGCCTTCTTTCGGGGAGGAAATTTTTATaATGGTGAATCATGATAACTTTACGAATCGCATGRTCTTGTAYCGGCGATAGTTCATTCAAATATCTGCCCTATCAACTTTCGATGGTAGTATAGAGGACTACCATGGTGGTAACGGGTAACGGAGAATCAGGGTTTGATTCCGGAGAAGGAGCCTGAGAAACGGCTACTACTTCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTAGTGACAAAAAATAACAATACCGGTCTTATTTAAGTCTGGTAATTGGAATGAGTACAATCTAAAACCTTTAACGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTTGATGTAACATATGATGACCCGCCTGTATAGGTGTGTTGTTGTTATATGTTCATCTCCTTACTAGACTT---TCATGRATGTCATTTGTTTGATGTTCATTGTA-TTCTGATTCGTCAGGGTGCGTGGTAGRAGGACCTATTGGGTTCGAGATTGTGGATGAATCTCTTCGGAGGTTGGTTCTGCTTGGATTCAGTAACCTTTTACTAGAAATCTAGTTCATTTACTTTGAGTAAATTAGAGTGTTCAAGGCAAGCAAACTT-GTTTCTGCAATGAATACTATTAGCATGGGATAATGGAACAGGACACGTATTCTAGTTTTCCGTTGGTTTCTAGAATTTTTGTACCYTTCGGRGTGCGTAGTGTAATGATTAAAAGGGACAGTTGGGGGCATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACTTCTGCGAAAGCATTTGTCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACCAGGGATTGGAAGATGTCTTATCCAGTG--TT--ACCCGCTGGAAAACTCCTTCAGTACCTTACGAGAAATCATAAGTCTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAAACTCACCAGGTCCAGACACAGTTAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGATAACGAACGAGACCTCAGCCTGCTAATTAGTGATGCAAATGATTACATCCGTAAGGAGAGGGTTCTTRTAAAAGCATTGRGTTTGACTTGGTGATTTTATRAGAAAACTTGTTTTCATTTGTTAGARTTTTGATACTTCGGTTGAMGAATTCGATAATGACTTCTTAGAGGGACACTTGGTGTTTAACCAAGGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATACGATCAACGAGTACTGAAGACCTGGTCCGAAAGGATTGGGTAAACTTTTGAAATCGTATCGTGCTGGGGATCGATAATTGTAATTATTTATCGTGAACGAGGAATTCCTAGTAAGCGCAAGTCAACAGCTTGCGCTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCTACCGATGTTTGGTCCGGTGAAATCTTAGGACTCGCGACTTTCGCTTGTTTTCCTCTCGGGGAGAATRAGTTTGTTGTGGGGAATTTGAGTAAACCTTGCTAAATAGAGGAAGCAAAAGTCGTAACAAGGTATTCGTAGGTGAACCTGCAGAAGGATCA
Importance of organism and transcriptomes	Neoparamoeba is one of the amoebozoan genera containing a ?parasome?, an obligate kinetoplastid symbiont located near the nucleus. It belongs to a group of amoebae, of which most of species were observed to be agents of mortal diseases of marine fishes and invertebrates. Transcriptome data available for Neoparamoeba may clarify the nature of the host-symbiont relationships in this system and contribute to understanding the evolution of Discosea.
Additional citations and references
Primary citation for organism's characterization, if available	Kudryavtsev A, Pawlowski J, Hausmann K (2011) Diversity of Amoebozoa from the deep-sea sediment samples of the Sea of Japan, in preparation
Latitude	42.26722
Longitude	136.71861
Depth (m)	3665
Salinity (psu)	35
Collection date	19-AUG-10
Collection time	08:25 PM
Sample collection site	Sea of Japan
Other collection site info	Expedition SoJaBio, bottom sediments of the Sea of Japan
Sample material (e.g. "seawater," "sediment," etc.)	Marine sediment
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Sediment
Habitat description	deep-sea bottom sediment, clay, aerobic
Other environmental metadata available	no other types of environmental metadata available
Other environmental metadata available	no other types of environmental metadata available
Habitat	marine habitat
Experimental Data
Date of experiment	18-MAY-11
Growth medium	seawater with wheat grains
Temperature (ºC)	20
Salinty (psu)	35
pH	7.5
Prey organism, if applicable (genus and species)	bacteria
Investigation type	Eukaryotes