Vannella robusta DIVA3 518/3/11/1/6

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Environmental Data
Principle Investigator(s)	Jan Pawlowski
External sample ID	V. robusta 518/3/11/1/6 Cysts
NCGR Sample ID	MMETSP0166
Sample accession number	CAM_SMPL_002442
Assembly accession number	CAM_ASM_000260
Combined Assembly Name	N/A
Genus	Vannella
Species	robusta
Strain	DIVA3 518/3/11/1/6
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	95228
18S rRNA	CTGGTTGATCCTGCCAGTAGTGATATGCTTGTTTTAAAGATTAAGCCATGCAAGTGCTAGTATAAATTGTTTTTACTGATGAAACTGCGAACGGCTCATTAAATCAGTTATAGTTTATTTGATGGTATTGAAGWWTTTCTTCAACTACATGGATAACTGTAGTAACCTAGAGCTAATACATGCATAACAATCAAACTATTTTGGAATGATTGGCACTTATTAGATATCAAACCAATAATCTTCGGATTAAATAAGGTGAATCATAATAACTGAACAGATCGCATGATTTTATATCGGCGACGTTTCATTCAAATTTCTGCCCTATCAACTAGATGTTAGTATATAGGACTAACATGGTTTTAACGGGTGACGGGGAATTGGGGTTCGATTCCGGAGAAGGAGCATGAGAAACGGCTACTACTTCTAAGGAAAGCAGCAGGCGCGCAAATTATTCAATGGAAACAAATTTCCGAAATAGTGACAAAAAATAACAATACATTGCATTAAATGTGATGTAATTGGAATGAGTACAATTTAAAAACCTTAACGAGTAACAATTAGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTAATAGCGTATATTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTGAATCTTGAATGTTCATYAAGTTTGGTTTACATTTTTATGTATAACTACTTGATTGCATTCTTCCTACTAGTAATTCGATACTGTACTTAATTGTATGGTATAGTGGTTCTTCGGRATCATCGTTATRRTGGAGTTGCTCCTCAAATAATTGRATTGGTGTAAAAGTCATGAAGATTATTTAGGGGAATCAGTAAATCTRTTATAATGGAATTCTAGTTCATTTACTTTGAGAAAATTAGAGTGTTTAAGGCAGCCTCTTGGGGCACTGAATATATTAGCATGGGATAATAAAATAGGATTCATATCATAAATTTGTTGGTTTGAATGATGTAAATAATGATTAATAGGGACAGTTGGGGGTATTTATATTGGATGGTTAGCGGTGAAATGCATGGATCCATTCAAGATAAACTAAAGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAGGTTAAGGGATCGAAGATGATTAGATACCGTCGTAGTCTTAACAGTAAACTATGCCGACTAGGGATATGAGATGTTATAAACATGTAACAGTGTTTAACTTCTTCTTGTGAACCTTGTGAGAAATCATAAGTTTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAGGGGCACCACCAGGAGTGGAGCGTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATTAGAAGGATTGACAGATTGATAGCTCTTTCATGATTTAATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGATAACGAACGAGACCTTTACCATACTAAATAGTATAATTTATYATTTTTRATAGATGTTAATACTTCTTAGAGGGACTATTGATTTTTAAATCAAAGGAAGTAAAGGCGATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACAATGATAGACTCAACGAGTATATAAATTTATTATTGCTTATCCGGAAGGATTGGCTAATCTTAAACGTTTATCGTGCTTGGGATAGATGCTTGCAATTATTTATCTTGAACCAGGAATTCCTAGTAATCGTATATCATCAGTGTGCGATGATTACGTCCCTGCCCCTTGTACACACCGCCCGTCGCTGCTACCGATTGGATGATCCGGTGAAATCTTCGGATAGCGAGAAATACTTTACTTTGTAAAGTTTCTTGTTAAAAGTTGATTAAACCTTATCATCTAGAGGAAGCAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATCA
Importance of organism and transcriptomes	Vannellid amoebae are one of the most important groups among Amoebozoa, as they are most widely distributed and most abundant in all habitats, including marine. The species nominated is one of the few cyst-forming vannellids, and one of the rare strains isolated from the deep-sea bottom. The obtained transcriptome data are pioneering for Vannella and will be used in comparison with the data on trophic amoebae to look for potential markers to distinguish trophic cells/cysts in metagenomic studies.
Additional citations and references
Primary citation for organism's characterization, if available	Kudryavtsev A, Pawlowski J, Hausmann K (2011) A survey of lobose amoebae (Amoebozoa) from the atlantic deep-sea sediments, in preparation
Latitude	-35.98833
Longitude	-49.0175
Salinity (psu)	35
Sample collection site	Atlantic_Ocean
Other collection site info	Expedition DIVA3
Sample material (e.g. "seawater," "sediment," etc.)	Marine sediment
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Sediment
Habitat description	abyssal plain bottom sediment, clay, aerobic
Habitat	marine habitat
Experimental Data
Date of experiment	04-JUL-11
Growth medium	seawater with wheat grains
Temperature (ºC)	20
Salinty (psu)	35
pH	7.5
Prey organism, if applicable (genus and species)	bacteria
Investigation type	Eukaryotes
Other experimental metadata available	RNA isolated from encysted (inactive) cells