Proboscia alata PI-D3

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Environmental Data
Principle Investigator(s)	Bank Beszteri
External sample ID	P. alata CTR 1
NCGR Sample ID	MMETSP0174
Sample accession number	CAM_SMPL_002451
Assembly accession number	CAM_ASM_000269
Combined Assembly Name	Proboscia-alata-PI_D3
Genus	Proboscia
Species	alata
Strain	PI-D3
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	216777
18S rRNA	attaacctggttttgtcctgccagtagtcatacgctcgtctcaaagattaagccatgcatgtctaagtgtaaatattttactttgaaactgcgaatggctcattatatcagttatagtttatttgatagtcccttactacttggatacccgtagtaattctagagctaatacatgcatcaatacccaacttatggaagggtagtatttattagattgaaaccaacccttttcggaggtattttggtgattcatgataatcatgcggattgcatggctttttgccggcgatggatcattcaagtttctgccctatcagctttggatggtagtgtattggactaccatggctttaacgggtaacggagggttagggctcgaccccggagagggagcctgagagacggctaccacatccaaggaaggcagcaggcgcgtaaattacccaatcctgatacagggaggtagtgacaataaataacaatgccgggccttttcaggtctggcaattggaatgagaacaatttaaatcccttatcgaggatccagcagagggcaagtctggtgccagcagccgcggtaattccagctctgctagcgtatattaaagttgttgcagttaaaaagctcgtagttgaacttctggtaggagtaattgttccatcactatgtgttggtaactgattgtctccggccatccttgggaaaagctcacatggcattaggttgtcgagtaagttgttcccatcgtttactgtgaaaaaattagagtgttcaaagcaggcttatgccattgaatatattagcatggaataataagataggaccttggtactattttgttggtttgcgcaccagggtaatgattaatagggacagttggtggtattcgtattcaattgtcagaggtgaaattcttggatttatggaagacgaactactgcgaaagcatttaccaaggatgttttcattaatcaagaacgaaagttaggggatcgaagatgattagataccatcgtagtcttaaccataaactatgccaactagggattggtggttgttattatgactccatcagcaccttctgagaaatcaaagtctttgggttccggggggagtatggtcgcaaggctgaaacttaaagaaattgacggaagggcaccaccaggagtggagcctgcggcttaatttgactcaacacgggaaaacttaccaggtccagacatagtgaggattgacagattgagagctctttcttgattctatgggtggtggtgcatggccgttcttagttggtggagtgatttgtctggttaattccgttaacgaacgagacccctgcctgctaaatagtcccgtgaatgaattttcattgatgaggtcttcttagagggacttttggtgcttaaccaaaggaagatgggggcaataacaggtctgtgatgcccttagatgtcctgggccgcacgcgcgctacactgatgcattcaacgagtatacaaccttggccgagagacctgggtaatcttttaaatgtgcatcgtgataggaatagattattgcaattattaatcttgaacgaggaattcctagtaaacgcagatcatcaatctgcattgattacgtccctgccctttgtacacaccgcccgtcgcacctaccgattgaatggtccggtgaagtctcgggattgtgatctggttcctttattggaacctgaccacgagaacttgcctaaaccttattatttagaggaaggtgaagtcgtaacaaggtttccgtaggtgaactgaaaaaaaggata
Importance of organism and transcriptomes	P. alata is a cosmopolitan species that can occasionally form large blooms. This transcriptome is part of a comparative study of the transcriptomic responses of Southern Ocean diatoms to iron limitation.
Additional citations and references
Primary citation for organism's characterization, if available	(Brightwell) Sundstrom 1986: 99, figs 258-266
Latitude	-64.0038
Longitude	-.0025
Collection date	20-JAN-08
Sample collection site	Southern_Ocean
Other collection site info	expedition ANT-XXIV / 2
Sample material (e.g. "seawater," "sediment," etc.)	seawater
ENVO term for habitat - primary term	Acquatic: marine
Other environmental metadata available	further metadata available at http://www.pangaea.de
Other environmental metadata available	further metadata available at http://www.pangaea.de
Habitat	marine habitat
Experimental Data
Date of experiment	26-MAY-11
Growth medium	modified f/2 amended seawater
Modifications to growth medium	Using trace metal solution after Morel et al. 1979. medium set up using trace metal clean conditions and procedures.
Temperature (ºC)	3
Salinty (psu)	33.6
pH	8.15
Light (µmol photons / m2 / sec)	35
Day portion of day:night cycle in hours	16
Night portion of day:night cycle in hours	8
Nitrate (μmol/L)	28
Ammonium (μmol/L)	.09
Phosphate (μmol/L)	28.45
Silicate (μmol/L)	60
Total Fe (nmol/L)	11000
Trace elements (total) (nmol/L)	7.3
Investigation type	Eukaryotes