Rosalina sp. Unknown

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Environmental Data
Principle Investigator(s)	Jan Pawlowski
External sample ID	65RSP
NCGR Sample ID	MMETSP0190_2
Sample accession number	CAM_SMPL_002445
Assembly accession number	CAM_ASM_000263
Combined Assembly Name	N/A
Genus	Rosalina
Species	sp.
Strain	Unknown
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	37353
18S rRNA	AAGGGCACCACAAGAACGCGTGGAGCATGTGGCTTAATTTGACTCAACGC GGGAAATCTTACCGGGTCCGGACACACTGATGATTGACAGGCAATATCAG CGCATCTCTGATGCTGCTGTGTTAAATATGCTAGTCCTTTCATGATTATG TGATAGGTGGTGCATGGCCGTTCTTAGTTCGTGGAGTGATCTGTCTGCTT AATTGCGTCTCACTAAGGAACTATATTTCTTATGTATGTTGTCAGCGCAT TGACCCCTCAACTGCTACAATTCTTAACTGAATTGACTTTTGCTGCGCGT GTCTTTGATTCTCGTCTGGCTCATACGATTAGTTCCTGAAAGCAACGAAC GTGACCGCAACCTCTTGTTGCCTTCAAATCATAATACATGCACTCATTTG GATGAATTTCATTTGTGTGCATACGGGAGGCTTTTCTAAACTAGAGGGAC CGCTGTTATCTTCTTTAGACCAGAGGAAAGGTTGCGGCAATAACAGG-TC TGTGATGCCCTTAGATGTTCCGGGCTGCACACGTGCTACAATGATTATTG CAGTGAGCATCTCATTTACTTAATACATCGCAAGCGCGAGTCCGTTTGTT CTTTGTTTCAGTTCATGCTGTTCAGAGCTTCAAATGAGTATCTCTGCGCG CGATAAAGCCTGCTTTGAAAGTTTAGTGGGTAATCAATTAGAAGTAATGA TTTCCTAAATTTATCGCACTTATATGTACGGCGTCTTTACCCAGCTGGCC TTTTGTGCCAGAATTCGTGTGTATCGACGATTGATTTCTTATAATCAATT GCCATTCTTACGGTACCGTACGTGCAATTGTCAATTCATGGTGGGGACAG ACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTTGTACGGG TCTTTGGTTCAACAGACCACCCGGAATACGTCCCTGCCCTTTGTACACAC CGCCCGTCGCTCTTACCGATGGACTTCTCTGTGAGTTTGAGGGACTGGGT CAATCTAAACTTCGCTTTAGANCTTTAAGACCTATGGAAACTTAAACGAA CAGTGTGNTNNNNNNNAAAGAGAAGTCGAACAAGGCA
Importance of organism and transcriptomes	Foraminifera are among the most abundant and diverse marine protists, characterized by granuloreticulopodia. Their tests or shells are an important component of marine sediments and widely used as biostratigraphic markers. Rosalina sp. is classified with the order Rotaliida for which no published EST exists up to date. The genus is common in the coastal waters and our strain was collected from the North Sea. To date, the molecular data on this organism, and foraminifers in general, is extremely poor represented in the available databases. Our knowledge on the gene expression repertoire and metabolic pathways used by foraminifers is still very limited. Additionally, transcriptomic data has shown to be a powerful tool for finding orthologous genes for further phylogenetic analysis, in an aim to resolve the phylogenetic relationships among the group and within the Eukaryotes.
Additional citations and references	i. Schweizer, M., Pawlowski, J., et al. (2008) Molecular phylogeny of Rotaliida (Foraminifera) based on complete small subunit rDNA sequences. Marine Micropaleontology 66(3-4): 233-246 ii. Pawlowski, J. (2009). Foraminifera, in: Moselio, S. (Ed). Encyclopedia of Microbiology. Oxford, Academic Press: 646-662. iii. Pawlowski, J., Bolivar, I., et al. (1994). Phylogenetic Position of Foraminifera Inferred from LSU rRNA Gene Sequences. Mol Biol Evol 11(6):929-938
Primary citation for organism's characterization, if available	Orbigny, A.D., Tableau methodique de la classe des Cephalopodes. 3eme ordre-Foraminiferes. Annales du Museum Naturelle, Paris, 1826, 7, 1-275.
Experimental Data
Date of experiment	25-MAR-11
Growth medium	1/8 Erdschreiber media plus filtered sea water
Temperature (ºC)	22
Salinty (psu)	35
pH	8
Day portion of day:night cycle in hours	10
Night portion of day:night cycle in hours	14
Nitrate (μmol/L)	12
Ammonium (μmol/L)	4
Phosphate (μmol/L)	2
Silicate (μmol/L)	1
Trace elements (total) (nmol/L)	15
Prey organism, if applicable (genus and species)	Chlorella sp., bacteria, diatoms