Alexandrium fundyense CCMP1719

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Environmental Data
Principle Investigator(s)	Senjie Lin
External sample ID	AlefuSL1
NCGR Sample ID	MMETSP0196C
Sample accession number	CAM_SMPL_002463
Assembly accession number	CAM_ASM_000281
Combined Assembly Name	Alexandrium-fundyense-CCMP1719
Genus	Alexandrium
Species	fundyense
Strain	CCMP1719
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	2932
18S rRNA	ATGTCTTAGTATAAGCTTCAATTTTGTGAAACTGCAAATGGCTCATTAAAACAGTCACAATGCATTTGGCGATCAATTCTAAATGGATATCTGTGGAAATTCTATAGTTAATACATGCACTAAAACTTATCTTTGGGGAAAGGTTGTGGTCGTTAGTTACAGAACCAATTCAAGCTATGCTTGGACACTTGAATGATTCACAATGACAAATGAATTACATGGCAACAGCTGGTGATAATTCATTCCAGTTTCTGACCTATCAGCTTTCGACGGTAAGGTATTGGCTTACCGTGGCAATGACAGGTGACGGAGGATTAGGGTTTGATTCCGGAGAGGGAGCTTGAGAAATGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGGCACAGGGAGGTAGTGACAAGAAATAACAATACAAGGCATCCATGTCTTGTACTTGGAATGAATGGATTTTAAACCTTTCTATAAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGCTGAGGATGGCTGGTCCGCCCTCTGGGTGAGTATTTGGCACAGCCTGAGCATTTATCTTGAAAGTACAACTGCACTTGACTGTGTGGTGTGTTATTGAGAACATTTACTTTGAGGAAATCAGAGTGTTTCAAGCAGGTGTTTGGCCTTGAATACATTAGCATGGAATAATAATCAAGATCGTGGTTCTTTTTTGTTGGTTTCTAGAATTGAGGTAATGATTAATAGGGATAGTTGGGGGCATTCGTATTTGATTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACGGACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAAGGGATCGAAGACGATCAGATACCGTCCTAGTCTTAACCATAAACCATGCCAACTAGAGATTGGAGGTTGTTACTTGTATGACTTTTTCAGCACCCTATGAGAAATCAAAGTGTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAATGAGGATTGACAGATTGATAGCTTTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACATGTAATTTCGATTATGTGGGCAACTTCTTAGAGGGACTTTGTGTGTATAATGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATGTGTTCAACGAGTTTTCAACCTTGCCTGGAAAGGTTTGGTAATCTTGAACAGGCATCGTGATGGGGATTGTTTATTGCAATTATTAACCTTCAACGAGGAATTCCTAGTAAGCTTGAGTCATCAGCTTGTGCTGATTATGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTTGGACTGTAGCAATGTTCAGTTCTTGAACAATGCAATGGCAAATTTAATGAACCTTATCACTTAGAGGAAGGAGAA
Importance of organism and transcriptomes	Alexandrium fundyense is a photosynthetic dinoflagellate that produces potent neuromuscular toxn (saxitoxin), which is accumulated in shellfish causing mortality of sea birds and mammals, and health concerns in humans. Blooms of this organism, most common in Gulf of Maine, New Hampshire, and Long Island Sound, causes dramatic economic loss. A. fundyense has typical peridinin-containing plastid, and its photosynthesis contributes to CO2 removal from the atmosphere and its conversion to organic matter for growth of its own and animals. A. fundyense is also bioluminescent, under the control of a luciferase-luciferin-binding protein enzyme system. Evolutionarily, Alexandrium is thought to be the most recently emerging lineage in dinoflagellates, calling for the need of in-depth phylogenetic/phylogeneomic analysis to verify this phylogenetic position.
Additional citations and references
Primary citation for organism's characterization, if available	Erdner, D. L., and D. M. Anderson. 2006. Global transcriptional profiling of the toxic dinoflagellate Alexandrium fundyense using massively parallel signature sequencing. BMC Genomics 7:88.
Latitude	43.1
Longitude	70.782
Sample collection site	Atlantic Ocean
Other collection site info	Portsmouth, New Hampshire
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	15-MAY-11
Growth medium	f/2 amended seawater
Temperature (ºC)	15
Salinty (psu)	28
pH	8
Light (µmol photons / m2 / sec)	200
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36.2
Trace elements (total) (nmol/L)	11.7
Investigation type	Eukaryotes
Other experimental metadata available	submitted is dinoflagellate spliced leader based cDNA prepared for culture enriched in G1 phase.