Marine Microbial Eukaryote Transcriptome Sequencing Project
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Thraustochytrium sp. LLF1b
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| Principle Investigator(s) | Eric E. Allen |
|---|---|
| External sample ID | LLF1b |
| NCGR Sample ID | MMETSP0198_2 |
| Sample accession number | CAM_SMPL_002465 |
| Assembly accession number | CAM_ASM_000283 |
| Combined Assembly Name | Thraustochytrium-sp-LLF1b |
| Genus | Thraustochytrium |
| Species | sp. |
| Strain | LLF1b |
| Clonal | Yes |
| Axenic | Yes |
| Prelim. NCBI Taxon ID | 1112570 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Thraustochytrids are prolific producers of hydrolytic enzymes and important degraders of particulate organic material in marine and estuarine environments. Chemical analyses of lipid production reveal that Thraustochytrium sp. LLF1b synthesizes significant quantities of the omega-3 polyunsaturated fatty acids docosahexaenoic acid (22:6) and eicosapentaenoic acid (20:5) important in metazoan health and development. Comparative analyses will significantly expand our knowledge of the genetic and metabolic potential of osmoheterotrophic protists in the marine environment and allow identification of genes associated with specific ecological niches. |
| Additional citations and references | |
| Environmental Data | |
| Latitude | 32.86667 |
| Longitude | 117.25 |
| Salinity (psu) | 33.4 |
| Temperature (ºC) | 15.2 |
| pH | 8.9 |
| Collection date | 07-APR-09 |
| Collection time | 02:45 PM |
| Sample collection site | Pacific_Ocean |
| Other collection site info | La Jolla Shores Beach |
| Sample material (e.g. "seawater," "sediment," etc.) | Ulva |
| Chlorophyll (mg/m3) | 2.7 |
| Nirate (μmol/kg) | 0.54 |
| Phosphate (μmol/kg) | 0.27 |
| Silicate (μmol/kg) | 8.3 |
| ENVO term for habitat - primary term | Organism-Associated |
| ENVO term for habitat - secondary term | Not Applicable |
| Habitat description | green alga surface (littoral zone) |
| Other environmental metadata available | no other types of environmental metadata available |
| Other environmental metadata available | no other types of environmental metadata available |
| Habitat | organism-associated habitat |
| Country | UNITED STATES |
| Experimental Data | |
| Date of experiment | 07-JUN-11 |
| Growth medium | B1 amended seawater |
| Modifications to growth medium | 5 g/L glucose |
| Temperature (ºC) | 25 |
| Salinty (psu) | 33.5 |
| pH | 7.4 |
| Nitrate (μmol/L) | 7 |
| Ammonium (μmol/L) | .28 |
| Phosphate (μmol/L) | 400 |
| Silicate (μmol/L) | 57 |
| Trace elements (total) (nmol/L) | 668 |
| Pressure (atm) | 1 |
| Investigation type | Eukaryotes |
| Other experimental metadata available | no other types of experimental metadata available |