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Karenia brevis Wilson

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Principle Investigator(s) Frances Van Dolah
External sample IDK. brevis Wilson log
NCGR Sample IDMMETSP0201
Sample accession numberCAM_SMPL_002468
Assembly accession numberCAM_ASM_000286
Combined Assembly NameKarenia-brevis-Wilson
GenusKarenia
Speciesbrevis
StrainWilson
ClonalUnknown
AxenicNo
Prelim. NCBI Taxon ID156230
18S rRNA
Importance of organism and transcriptomesKarenia brevis is a planktonic dinoflagellate that produces harmful algal blooms almost every year in the Gulf of Mexico. Most strains of K. brevis produce a suite of toxic compounds including brevetoxins (2 main backbone types, PbTx-1 and -2) that are potent neurotoxins. The dominant life history stage of dinoflagellates is haploid, and the sexual stages and resting cysts (if any) are not well known for K. brevis. Most clonal cultures of K. brevis produce ~10-20 pg brevetoxin per cell and this is mostly PbTx-2 (90% of total toxin). The SP1 clone produces extremely low levels of brevetoxin (or, none). The toxin synthesis pathway and the function of brevetoxin in K. brevis is not known, therefore transcriptome data for SP1 clone in comparison with data from other clones (such as SP3, which produces mostly PbTx-1 and Wilson, which produces consistly high levels) will be informative. The comparative approach is valuable as no experimental genetic system (e.g. knock-out mutants) is available. Karenia brevis is a planktonic dinoflagellate that produces harmful algal blooms almost every year in the Gulf of Mexico. Most strains of K. brevis produce a suite of toxic compounds including brevetoxins (2 main backbone types, PbTx-1 and -2) that are potent neurotoxins. The dominant life history stage of dinoflagellates is haploid, and the sexual stages and resting cysts (if any) are not well known for K. brevis. Most clonal cultures of K. brevis produce ~10-20 pg brevetoxin per cell and this is mostly PbTx-2 (90% of total toxin). The SP1 clone produces extremely low levels of brevetoxin (or, none). The toxin synthesis pathway and the function of brevetoxin in K. brevis is not known, therefore transcriptome data for SP1 clone in comparison with data from other clones (such as SP3, which produces mostly PbTx-1 and Wilson, which produces consistly high levels) will be informative. The comparative approach is valuable as no experimental genetic system (e.g. knock-out mutants) is available.
Additional citations and referencesMorey, J.S., Monroe, E.A., Kinney, A.L., Beal, M., Johnson, J.G., Hitchcock, G.L., Van Dolah, F.M. 2011. Transcriptomic response of the Florida red tide dinoflagellate, Karenia brevis, to nitrogen and phosphorus depletion and addition. BMC Genomics (in press)
Environmental Data
Primary citation for organism's characterization, if availableDaugbjerg, N., Hansen, G., Larson, J., Moestrup, O. 2000. Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmored dinoflagellates. Phycologia 39: 302-317.
Collection date01-JAN-53
Sample collection siteGulf of Mexico
Other collection site infoSt. Johns Pass, FL
ENVO term for habitat - primary termAcquatic: marine
Habitat descriptioncoastal harmful algal bloom
Habitatmarine habitat
CountryUNITED STATES
Experimental Data
Date of experiment07-APR-08
Growth mediumf/2 amended seawater
Temperature (ºC)25
Salinty (psu)36
pH8.3
Light (µmol photons / m2 / sec)50
Day portion of day:night cycle in hours16
Night portion of day:night cycle in hours8
Nitrate (μmol/L)883
Phosphate (μmol/L)36
Trace elements (total) (nmol/L)25
Investigation typeEukaryotes
Other experimental metadata availablemid-log phase, division rate 0.6 div./day