Karenia brevis Wilson

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Environmental Data
Principle Investigator(s)	Frances Van Dolah
External sample ID	K. brevis Wilson log
NCGR Sample ID	MMETSP0201
Sample accession number	CAM_SMPL_002468
Assembly accession number	CAM_ASM_000286
Combined Assembly Name	Karenia-brevis-Wilson
Genus	Karenia
Species	brevis
Strain	Wilson
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	156230
18S rRNA	GAAACTGCGAATGGCTCATTAAAACAGTTATAGTTTATTTGATGGTCATTCATTACATGGATAACTTTGGAAATTCTAGAGCTAATACATGCGCCCAAACCCGACTTCTTGGAAGGGTTGTGTTTATTAGATACAGAACCAACCCAGGCCCTGCCTGGTCTTGTGGTGATTCATAATAACCGAACGAATCGCATTGCATCAGCTGGCGATAGATCATTCAAGTTTCTGACCTATCAGTTTCCGACGGTAGGGTATTGGCCTACCGTGGCATTGACGGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGAATGAGTAGAATTTAAATCCCTTTGCGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGCCGAGGACGACCGGTCCGCCCTCTGGGTGAGTATCTGGCTCGGCTTGGGCATCTTCTTGGAGAACGTAGCTGCACTTGACTGTGTGGTGCGGTATCCAGGACTTTTACTTTGAGGAAATTAGAGTGTTTCAAGCAGGCATACGCCTTGAATACATTAGCATGGAATAATAAGATAGGACCTCGGTTCTATTTTGTTGGTTTCTAGAGCTGAGGTAATGATTAATAGGGATAGTTGGGGGCATTCGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACGGACTACTGTGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCCTAGTCTTAACCATAAACCATGCCGACTAGAGATTGGAGGTCGTTACTTATACGACTCCTTCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGTGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACACGTAACTTCGGTTACGTGGGCAACTTCTTAGAGGGACTTTGCGTGTCTAACGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATGCGCTCAACGAGTTTTCGACCTTGCCCGGAAGGGTTGGGTAATCTTTTTAAAACGCATCGTGATGGGGATAGATTATTGCAATTATTAATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGTGCTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCCGCAGTGTTCAGATCCTGAACGTTGCAGGGAAAGTTTAGTGAACCTTATCACTTAGAGGAAGGAGAAGTC
Importance of organism and transcriptomes	Karenia brevis is a planktonic dinoflagellate that produces harmful algal blooms almost every year in the Gulf of Mexico. Most strains of K. brevis produce a suite of toxic compounds including brevetoxins (2 main backbone types, PbTx-1 and -2) that are potent neurotoxins. The dominant life history stage of dinoflagellates is haploid, and the sexual stages and resting cysts (if any) are not well known for K. brevis. Most clonal cultures of K. brevis produce ~10-20 pg brevetoxin per cell and this is mostly PbTx-2 (90% of total toxin). The SP1 clone produces extremely low levels of brevetoxin (or, none). The toxin synthesis pathway and the function of brevetoxin in K. brevis is not known, therefore transcriptome data for SP1 clone in comparison with data from other clones (such as SP3, which produces mostly PbTx-1 and Wilson, which produces consistly high levels) will be informative. The comparative approach is valuable as no experimental genetic system (e.g. knock-out mutants) is available. Karenia brevis is a planktonic dinoflagellate that produces harmful algal blooms almost every year in the Gulf of Mexico. Most strains of K. brevis produce a suite of toxic compounds including brevetoxins (2 main backbone types, PbTx-1 and -2) that are potent neurotoxins. The dominant life history stage of dinoflagellates is haploid, and the sexual stages and resting cysts (if any) are not well known for K. brevis. Most clonal cultures of K. brevis produce ~10-20 pg brevetoxin per cell and this is mostly PbTx-2 (90% of total toxin). The SP1 clone produces extremely low levels of brevetoxin (or, none). The toxin synthesis pathway and the function of brevetoxin in K. brevis is not known, therefore transcriptome data for SP1 clone in comparison with data from other clones (such as SP3, which produces mostly PbTx-1 and Wilson, which produces consistly high levels) will be informative. The comparative approach is valuable as no experimental genetic system (e.g. knock-out mutants) is available.
Additional citations and references	Morey, J.S., Monroe, E.A., Kinney, A.L., Beal, M., Johnson, J.G., Hitchcock, G.L., Van Dolah, F.M. 2011. Transcriptomic response of the Florida red tide dinoflagellate, Karenia brevis, to nitrogen and phosphorus depletion and addition. BMC Genomics (in press)
Primary citation for organism's characterization, if available	Daugbjerg, N., Hansen, G., Larson, J., Moestrup, O. 2000. Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmored dinoflagellates. Phycologia 39: 302-317.
Collection date	01-JAN-53
Sample collection site	Gulf of Mexico
Other collection site info	St. Johns Pass, FL
ENVO term for habitat - primary term	Acquatic: marine
Habitat description	coastal harmful algal bloom
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	07-APR-08
Growth medium	f/2 amended seawater
Temperature (ºC)	25
Salinty (psu)	36
pH	8.3
Light (µmol photons / m2 / sec)	50
Day portion of day:night cycle in hours	16
Night portion of day:night cycle in hours	8
Nitrate (μmol/L)	883
Phosphate (μmol/L)	36
Trace elements (total) (nmol/L)	25
Investigation type	Eukaryotes
Other experimental metadata available	mid-log phase, division rate 0.6 div./day