Euplotes focardii TN1

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Environmental Data
Principle Investigator(s)	Denis Lynn
External sample ID	EFTR4
NCGR Sample ID	MMETSP0205
Sample accession number	CAM_SMPL_002460
Assembly accession number	CAM_ASM_000278
Combined Assembly Name	Euplotes-focardii-TN1
Genus	Euplotes
Species	focardii
Strain	TN1
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	36767
18S rRNA	tagtcatacg cttgtctcaa agattaagcc atgcatgtct aagtataaag gttacataca atgaaactgc gaatggctca ttcaaacagt tatagtttat ttggattaaa acatttgttt aaatggataa ccgtagtaat tctagggcta atacatgcgt tacgagggac tttacggaac ctcagtattt attagatttc aaaccaatat tccgcaaggt ctactgagat gattcatgat aacttatcgg attgctgggt ctaccggcaa tgaaccattc atgtttctgc ttcccatcag cttgatggta gtgtattgga caaccatggc attcacgggc tatcggggga ttagggttcg attccggaga gggagcctga gaaacggcta ccacttctac ggaaggcagc aggcgcgaaa attatccaat cctgattcag ggaggtagtg aaagaaataa tgaactagga tttatcctgg ggtcacaatg ggcttgattt gcaaactttc tagcgaggaa caattggagg gcaagtctgg tgccagcagc cgcggtaatt ccagctccaa tagtgtatat taatgttcct gcagttattc gatgctcgta gttggatttc tggaggctga gacaggaggg cagccaaggt tgtcgccagt ctcttccttc atccgcctgt taacgttgtc cgggatttat ttctcggctt cgggctcagg tacttttttt gttttaccct ttctttatat tcatttattt atctttattt tttgagcaaa ttatagtgtt tcaggcaggc gtgcgccgga atacattagc atggaataat cgaattggac cgtgtattca ctatgtgaat tcttccttat ttgttggttc taaggacacg gatatgatta atagggatag taattttcag gggaggcatt agtatttaat ttccagaggt gaaattcttt gaaatattaa agactaactt atgcgaaagc atttattttg ccaataatgt tttcattaat cattgaacga aagttagggg atcaaagacg atcagatacc gtcctagtct taaccataaa cgttgccgac tagggatcgg agggcgtgca cattccgcct tcggcacctt acgagaaatc aaagtctttt gggttctggg ggtagtatgg tcgcaaggct gaaacttaaa ggaattgacg gaagggcacc accaggagtg gagcttgcgg ctcaatttga ctcaacacgg gaaatcttac caggtccaga catagcgagg attgacagat tgatagctct ttcttgattc tatgggtatt tttggtggtg catggccgtt cttagttggt ggagtgattt gtctggttaa ttccgttaaa cgaacgagac ctcagcctgc taaatagtta cctgtctttt cttatgagac ttgataactt cttagaggga cgttgtgtgc aaccacaagg aagttgaggc aataacaggt ctgtgatgcc cttagatgtc ctgggccgca cgcgtgctac actgatacgt acaacgaggt atatgcattt atgcatcttt gctgctccga aatagacaca gctaaatctt ctaaaatacg tatcgtgctg cggatagatc gttgaaatta tggatcttga aggtggaatt cctagtaagc gcaggtcatc aacctgcgtt gattacgtcc ctgccctttg tacacaccgc ccgtcgctcc taccaatttc gagtggctcg gtgaacctct ttggactgtc gagcaatcgc gaaattagag tgaacctggt cacttagagg aaggagaagt cgtaacaagg tttccgtagg t
Importance of organism and transcriptomes	###############################################################################################################################################################################################################################################################
Additional citations and references	###############################################################################################################################################################################################################################################################
Primary citation for organism's characterization, if available	Pucciarelli, S, La Terza, A, , Ballarini, P., Barchetta, S, Yu, T, Marziale, F, Passini, V, Methe, Detrich, HW III, Miceli, C. 2009. Molecular cold-adaptation of protein function and gene regulation: the case for comparative genomic analyses in marine ciliated protozoa. Marine Genomics 2 (2009) 57?66
Collection date	01-JAN-88
Sample collection site	Terra Nova Bay
Sample material (e.g. "seawater," "sediment," etc.)	seawater and sediment trap material
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Sediment
Habitat	marine habitat
Country	ANTARCTICA
Experimental Data
Date of experiment	09-MAY-11
Growth medium	Cells were grown in natural sea water collected in Adriatic Sea. Twice per week 50ml of the algae Dunaliella tertiolecta grown in Walne's medium (http://www.ccap.ac.uk/media/recipes/Walnes.htm) were added to each one-liter cell cultures
Temperature (ºC)	4
Salinty (psu)	35
pH	8
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	300
Ammonium (μmol/L)	100
Phosphate (μmol/L)	50
Silicate (μmol/L)	100
Trace elements (total) (nmol/L)	750
Investigation type	Eukaryotes