Condylostoma magnum COL2

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Environmental Data
Principle Investigator(s)	Denis Lynn
External sample ID
NCGR Sample ID	MMETSP0210
Sample accession number	CAM_SMPL_002484
Assembly accession number	CAM_ASM_000302
Combined Assembly Name	N/A
Genus	Condylostoma
Species	magnum
Strain	COL2
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	40633
18S rRNA	TAGTCATATGCTTGTCTTAAAGATTAAGCCATGCATGTCTAAGTATAAGCTTTTATACAG TGAGACTGCGAATGGCTCATTAAATCAGTTATAGTTTATTTGCTGAACGTTTATATGGAT AACCGTAGTAATTCTAGAGCTAATACATGCTGGTTGCGCAGTGCGTAGTATATATTAGAT AAAGCCAACGGGCGAGGAGTCATAATATCTGAGCGGACTTCGTTTGAAGTGCAGCATTGA AGTTTCTGCCCTATCAGCTTGATGGTAGTGTATTGGACTACCATGGCAATGACGGGTGAC GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAA GGCAGCAGGCGCGCAAATTACCCAATCCTAACTCAGGGAGGTAGTGACAAGAAATAACAA CGCGGGGCAAAGTCTCGTGATTGGAATGAGGACAATGTAAAAGCCTTATCGAGGATCCACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATACCAGCT CCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAAGTTCTGTACGGA TTGAGTCTCYACGGAGACACAAGCCGTACATCCGTCTGATTCTTCAGACACTTTACCTTG AGCAAATTAGAGTGTTCCAGGCAGGCGCCAGCCTGAATAGTCCAGCATGGAATAATAGAA AAGGACGAAGAGCCACTGTTGGTGCGTGGCCCTTAGTAATGATTAATAGGGATAGTTGGG GGCATTCGTATTTAATTGTCAGAGGTGAAATTCTATGATTTATTAAAGACGAACCTATGC GAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAAGGGATCAAAGACG ATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGAGATTGGATGGGGAATT CAAGACCCATTCAGGATCTTCCGAGAAATCAAAGTCTTTGGGTTCTGGGGGGAGTATGGT CGCAAGACTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGTGTGGAGCCTGCGGC TTAATTTGACTCAACACGGGGAAACTTACCAGGGCCAGACATGGCAAGGATTGACAGACTGACAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT TGGTGGAGTGATTTGTCTGGTTAATTCCGATAACGAACGAGACCTCAGCCTGCTAACTAG TTCGTTCCTGCTTAGGATCGGGACTTCTTAGAGGGACTTTGGGGGTTACTCCAAGGAAGT TTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCCGCACGCGCGCTACACT GATGCAGGCAGTAAGTTCCATGTCAGACATGTCATGGGAACCTTCAAACTGCATCGTGAT GGGGATTGACTCTTGGAATTCTTGGTCATAAACGAGGAATACCTAGTAAACGCAAGTCAT CAGCTTGCATTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTG AGTGGTGAGGTGAACCCTTCGGACCGCTTACCCGGGAAGTTGTGTAAACCTTACCACTTA GAGGAAGGAGAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATCA
Importance of organism and transcriptomes	###############################################################################################################################################################################################################################################################
Additional citations and references
Primary citation for organism's characterization, if available	Modeo L., Dias R. J., Fernandes N., Tomei G., Boscaro V., Verni F., da Silva-Neto I. D., Petroni G. (2011). Phylogenetic systematics of the genus Condylostoma (Ciliophora, Heterotrichea) by means of a multidisciplinary analytical study of some marine and brackish morphospecies. VI European Congress of Protistology (ECOP), 24-29 July 2011. Berlin. Final Programme and Abstracts, p. 91.
Latitude	43.3
Longitude	10.2
Salinity (psu)	33
Collection date	15-MAR-07
Collection time	10:00 AM
Sample collection site	Ligurian Sea
Other collection site info	Naval Academy, Livorno (Tuscany)
Sample material (e.g. "seawater," "sediment," etc.)	seawater and sediment
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Other
Habitat description	50-100 cm diameter, 5-10 cm deep, pools on a rocky shore near Livorno, Ligurian Sea
Other environmental metadata available	no other types of environmental metadata available
Other environmental metadata available	no other types of environmental metadata available
Habitat	marine habitat
Country	ITALY
Experimental Data
Date of experiment	20-JAN-12
Growth medium	autoclaved artificial seawater (Red Sea Salt, Red Sea, http://www.redseafish.com)
Temperature (ºC)	19
Salinty (psu)	33
pH	8.2
Light (µmol photons / m2 / sec)	300
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Prey organism, if applicable (genus and species)	Pheodactylum tricornutum
Investigation type	Eukaryotes
Other experimental metadata available	Cells treated with 0.1 mg/ml ampicillin overnigth and then washed in autoclaved seawater