Euplotes harpa FSP1.4

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Environmental Data
Principle Investigator(s)	Denis Lynn
External sample ID
NCGR Sample ID	MMETSP0213
Sample accession number	CAM_SMPL_002470
Assembly accession number	CAM_ASM_000288
Combined Assembly Name	N/A
Genus	Euplotes
Species	harpa
Strain	FSP1.4
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	151035
18S rRNA	tagtcatacg cttgtctcaa ggattaagcc atgcatgtct aagtataaag gtacatacaa tgaaactgcg aatggctcat tcaaacagtt atagtttatt tgatattcaa actaatgaaa gttagtttaa atggataacc gtagtaattc tagagctaat acatgcgtta cgggggactt tacggaaccc cagtatttat tagattcaaa ccaatattcc gcaaggtcta ctgagatgat tcatgataac tgatcgaatt gctgggtcta ctagcaataa atcattcatg tttctgcttc ccatcagctt gatggtagtg tattggacaa ccatggcatt cacgggctat cgggggatta gggttcgatt ccggagaggg agcctgataa acggctacca cttctacgga aggcagcagg cgcgaaaatt atccaatcct gactcaggga ggtagtgaaa gaaataatga actaggattt atcctgggat cacaatgggc ttgatttgca aactttacat ttagcgagga acgattggag ggcaagtctg gtgccagcag ccgcggtaat tccagctcca atagtgtata ttaatgttcc tgcagttatt cgatgctcgt agttggattt ctgaagttga gatgggaggg tggctatggc catcgctcgt ctcttccttc atccacctgt tagcgaatcc cgggattcac ttctcggttt cgggctcagg tactttacca tttttatata cctatattta tatcctgtag tctttatcga ttatgtgtta tttcttcttt gagcaaatta tagtgtttca ggcaggcgtg cgccggaata cattagcatg gtataatcga attggaccgt gtatccatta tttggattcc tccttatttg ttggtttaaa ggacacggaa atggttaata gggatagtat ttaatccagg ggaggcatta gtatttaatt tccagaggtg aaattctttg aaatattaaa gactaactta tgcgaaagca tttattttgc caataatgtt ttcattaatc attgaacgaa agttagggga tcaaagacga tcagataccg tcctagtctt aaccataaac gttgccgact agggatcgga gggcgtgcac attccgcctt cggcacctta cgagaaatca aagtcttttg ggttctgtgg gtagtatggt cgcaagactg aaacttaaag gaattgacgg aagggcacca ccaggagtgg agcttgcggc tcaatttgac tcaacacggg aaatcttacc aggtccagac atagcgagga ttgacagatt gatagctctt tcttgattct atgggtattt ttggtggtgc atggccgttc ttagttggtg gagtgatttg tctggttaat tccgttaaac gaacgagacc tcagcctgct aaatagttac ctgtctctct tacgagactt gataacttct tagagggact ttgtgtgcaa ccacaaggaa gtttgaggca ataacaggtc tgtgatgccc ttagatgtcc tgggccgcac gcgtgctaca ctgatacgta caacgaggta tatgtacttg tacatcaatg ctgctccgaa atagacacag cttaatcttc taaaatacgt atcgtgctgc ggatagatcg ttgaaaattt tataataaca aattatgaat cttgaaggtg gaattcctag taagcgcagg tcattagcct gcgttgatta cgtccctgcc ctttgtacac accgcccgtc gctcctacca atttcgagtg gtttggtgaa cctctttgga ctgtcgagca atcgcgaaat tagagtgaac ctgattactt agaggaagga gaagtcgtaa caaggtttcc gtaggt
Importance of organism and transcriptomes	###############################################################################################################################################################################################################################################################
Additional citations and references	###############################################################################################################################################################################################################################################################
Primary citation for organism's characterization, if available	Vannini C., Petroni G., Verni F. & Rosati G. 2005. Polynucleobacter bacteria in the brackish?water species Euplotes harpa (Ciliata, Hypotrichia). J. Eukaryot. Microbiol., 52(2): 116-122.
Latitude	43.47
Longitude	10.16
Salinity (psu)	5
Collection date	15-SEP-00
Sample collection site	estuary of Serchio River (Pisa)
Sample material (e.g. "seawater," "sediment," etc.)	Brackish water and sediment
ENVO term for habitat - primary term	Other
ENVO term for habitat - secondary term	Other
Habitat description	Brackish water estuarine pond
Habitat	other
Country	ITALY
Experimental Data
Date of experiment	01-FEB-12
Growth medium	autoclaved artificial seawater (Red Sea Salt, Red Sea, http://www.redseafish.com) diluted with distilled water
Temperature (ºC)	19
Salinty (psu)	5
pH	8.2
Light (µmol photons / m2 / sec)	300
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Prey organism, if applicable (genus and species)	Dunaliella salina and Dunaliella tertiolecta
Investigation type	Eukaryotes
Other experimental metadata available	Cells treated with 0.1 mg/ml ampicillin overnigth and then washed in autoclaved seawater. Cells harbour obligate endosymbionts of the species Polynucleobacter necessarius (Betaproteobacteria) ampicillin-resistent.