Akashiwo sanguinea CCCM 885

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Environmental Data
Principle Investigator(s)	Juan Saldarriaga
External sample ID	Aksang
NCGR Sample ID	MMETSP0223_2
Sample accession number	CAM_SMPL_002482
Assembly accession number	CAM_ASM_000300
Combined Assembly Name	N/A
Genus	Akashiwo
Species	sanguinea
Strain	CCCM 885
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	143672
18S rRNA	CCGAATTCGTCGACAACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCAT GTCTCAGTATAAGCTTCTATATGGCAAAACTGCGAATGGCTCATTAAAACAGTTATAGTTCATTTGATGG TCTCTCACTACATGGATAACTGTGGTAATTCTAGAGCTAATACATGCGCCCTAACCCGACTTCGTAGAAG GGTTGTGCTTATTAGACACAGAACCAACCCAGGCTCTGCCTGGTCTTGTGGTGAGTCATAATAACTGAAC GAATCGCATGGCATCGCCGGCGATAAATCATTCAAGTTTCTGACCTATCAGCTTCCGACGGTAGGGTATT GGCCTACCGTGGCAATGACGGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC TACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTAGTGACAAGAAA TAACAATACAGGGCATCCATGTCTTGTAATTGGAATGAGTAGAATTTAAATCCCTTTACGAGTATCGATT GGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCGGT TAAAAAGCTCGTAGTTGGATTTCTGCCGAGAACGACCGGTCCGCCCTCTGGGTGAGTATCTGGCTCGGTC TGGGCATCTTCTTGGAGAACGTAGCTGCACTTGACTGTGTGGTGCGGTATCCAGGACATTTACTTTGAGG AAATTAGAGTGTTTCAAGCAGGCATGTGCCTTGAATACATTAGCATGGAATAATAATATAGGACCTTAAT TCTATTTTGTTGGTTTCTAGAGTTGAGGTAATGATTAATAGGGATAGTTGGGGGCATTCGTATTTAACTG TCAGAGGTGAAATTCTTGGATTTGTTAAAGACGGACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATT GATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCCTAGTCTTAACCATAAACCATGCCG ACTAGAGATTGGAGGTCGTTATCATTACGACTCCTTCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTC CGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGCGTGGAGCCT GCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTGATAG CTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATT CCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACACGTAACTCCGGTTATGTGGGCAACTTCTTAG AGGGACTTTGTGTGTCTAACGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTG GGCTGCACGCGCGCTACACTGATGCGGTCAACGAGTTTCTGCCTGGTCCGGCAGGATTGGGTAATCTTCT TAAATCGCATCGTGATGGGGATAGATTATTGCAATTATTAATCTTCAACGAGGAATGCCTAGTAAGCGCG AGTCATCAGCTCGTGCTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAGTG ATCCGGTGAATAATTCGGACCAACCCAGGGCCCAGTTACTGTGTGCTGGGGTGGAAAGTTTAGTGAACCT TATCACTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATCAAGCTTGGA TCCCGGG
Importance of organism and transcriptomes	Akashiwo sanguinea is a common bloom-forming dinoflagellate that has been implicated in fish kills. It is at best a nuisance organism, but in 2009 it was shown to produce proteinaceous surfactants that caused sea bird feathers to saturate with water; many birds died subsequently of hypothermia (Jessup et al. 2009). Morphologically it is very much gymnodinialean, but phylogenetic trees based on rRNA genes have not been able to place the genus with any degree of certainty. Nevertheless it does not seem to be a member of the Gymnodinium clade.
Additional citations and references	Jessup, David A.; Miller, Melissa A.; Ryan, John P.; Nevins, Hannah M.; Kerkering, Heather A.; Mekebri, Abdou; Crane, David B.; Johnson, Tyler A.; Kudela, Raphael M. (2009): Mass-stranding of marine birds caused by a surfactant-producing red tide. PLoS One 4: e4550
Primary citation for organism's characterization, if available	Daugbjerg, N., Hansen, G., Larsen, J. & Moestrup, ?. (2000). Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmoured dinoflagellates. Phycologia 39: 302-317.
Latitude	40.6666
Longitude	-73.25
Collection date	20-JUL-58
Sample collection site	Great South Bay, Long Island, New York
Sample material (e.g. "seawater," "sediment," etc.)	seawater
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	02-JUN-11
Growth medium	HESNW
Modifications to growth medium	no silica
Temperature (ºC)	18
Salinty (psu)	30
pH	7
Light (µmol photons / m2 / sec)	3800
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	3
Ammonium (μmol/L)	.11
Phosphate (μmol/L)	.3
Investigation type	Eukaryotes