Togula jolla CCCM 725

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Environmental Data
Principle Investigator(s)	Juan Saldarriaga
External sample ID	Togjoll
NCGR Sample ID	MMETSP0224
Sample accession number	CAM_SMPL_002471
Assembly accession number	CAM_ASM_000289
Combined Assembly Name	N/A
Genus	Togula
Species	jolla
Strain	CCCM 725
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	285029
18S rRNA	AGTCATATGCTTGTCTCAAAGACTAAGCCATGCATGTCTCAGTATAAGCCTCTACATGGTGAAACTGCAA ATGGCTCATTAAAACAATTATGGTTTACTTGATGATCAACACCTACATGGATAACTGTGGTAATTCTATA GCTAATACTTGCGCTCAAGCCCGACTTTGTGGAAGGGTTGTGATTATCAGGTACAGAACCATCCCAGGCC ATGCCTGGACATATGGTGACTCATGGTGACCAAACGAATTTCGTGGCATATGCCGGCAATGTGTCATTCG AGTTTCTGACCTATCAGCTTCCGACGGTAGGGTATTGGCCAACCGTGGCAATGACGGGTAACGGAGAATT AGGGTTCGATTCCGGAGAGGGAGCCTGAGAAATGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAAT TACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGA ATGAGCAAAATTTACATCTCTCTGTGAGTACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAAT TCCAGCTCCAATAGCGTATATTAAAGCTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGCCGAGGACA ACCGGTCCGCCCTCCGGGTGAGTATCTGGATTGACCTGGGCATCTTCTTGGGGAATGTAGCTGCACTTGA TTGTGTGACGCAGGATTCAATACTTTTACTTTGAAAAAATTAGAGTGTTTCACGCAGGCGTATGCCTTGA ATACATTAGCATGGGATAATGAAATGGGACCTTGGTTCTTTCTTGTTGGTTTCTAGAGCAGAGGTAAGGA CAAATAGGGATAGCTGGGGGTATTTGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGATGG ACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGAT CAGATACCGTCCTAGTCTTAACCATAAACCATGCCAACTAGAGATTGAAGGTCGTTACCTACACGACCCC CTCAGGACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAA GGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTAC CAGGTCCAGACATAGTAAGGATTGACAGATTGACAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGC CGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACTTACTAAATCG TTACGCGTAGCTTTGGTTACATGGGCAACTTCTTAGAGGGACTTTGTGTGTCTAACACAGGGAAGTTTGA GGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATGCGCTCAACGA GTTTTTGACCTTACCTGACAAGGATGGGTAATCTCTCCAGAATGCATCGTGATGGGGATAGTTTTTTGCA ATTATTAATCTTGAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGTGCTGATTATGTCCCTGCCC TTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGAATACTTTCGACCGTGGCAGCACTT GGTTCCTGAGTGGGGCAATGGAAAGTTGAGTGAACCTTATCACTTAGAGGAAGGAGAAGTCGTAACAAGG TTTCC
Importance of organism and transcriptomes	Togula jolla is a common dinoflagellate in benthic, temperate intertidal environments. Morphologically it is very much a gymnodinialean, but phylogenetic trees based on rRNA genes have not been able to place the genus with any degree of certainty. Nevertheless it does not seem to be a member of the large Amphidinium clade. The strain proposed to be used for this study is one of the ones used for the description of the species.
Additional citations and references
Primary citation for organism's characterization, if available	J?rgensen, M.F., Murray, S. & Daugbjerg, N. (2004). A new genus of athecate interstitial dinoflagellates, Togula gen. nov., previously encompassed within Amphidinium sensu lato: Inferred from light and electron microscopy and phylogenetic analyses of partial large subunit ribosomal DNA sequences. Phycological Research 52(3): 284-299
Sample material (e.g. "seawater," "sediment," etc.)	seawater
Habitat	marine habitat
Experimental Data
Date of experiment	24-JUN-11
Growth medium	HESNW
Modifications to growth medium	no silica
Temperature (ºC)	18
Salinty (psu)	30
pH	7
Light (µmol photons / m2 / sec)	3800
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	3
Ammonium (μmol/L)	.11
Phosphate (μmol/L)	.3
Investigation type	Eukaryotes