Thoracosphaera heimii CCCM 670 (=CCMP 1069)

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Environmental Data
Principle Investigator(s)	Juan Saldarriaga
External sample ID	Thorheim
NCGR Sample ID	MMETSP0225
Sample accession number	CAM_SMPL_002472
Assembly accession number	CAM_ASM_000290
Combined Assembly Name	N/A
Genus	Thoracosphaera
Species	heimii
Strain	CCCM 670 (=CCMP 1069)
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	2923
18S rRNA	AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTGAAATTAGCTTCTGAACGGTGAATTTGCAAA TGGCTCATTACAACAGTTATGGTTTATTTGATGGTCATTCATTACATGGATAACTGTGGTAATTCTAGAG CTAATACATGCGCCCAAACCCGACTCCGTGGAAGGGTTGTGTTTATTAGTTACAGAACCAACCCAGGCTC CGCCTGGTTTTTTGGTGATTCATAATAACCGAACGAATCGCATGGCATCAGCCGGCGATGAATCATTCAA GTTTCTGACCTATCAGCTTCCGACGGTAGGGTATTGGCCTACCGTGGCAATGACGGGTAACGGAGAATTA GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATT ACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGAA TGAGTAGAATTTAAATCCCTTTATGAGTATCGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATT CCAGCTCCAATAGCGTATATTTAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGACTTCTGCCGAGGACGA CCGGTCCGCCCTCTGGGTGAGTATCTGGCTCGGCCTGGGCATCTTCTTGGAGAACGTATCTGCACTTGAC TGTGTGGTGCGGTATCCAAGACTTTTACTTTGAGGAAATTAGAGTGTTTCAAGCAGGCACACGCCTTGAA TACATTAGCATGGAATAATAAGATAGGACCTTGGTTCTATTTTGTTGGTTTCTAGAGCTGAGGTAATGAT TAATAGGGATAGTTGGGGGCATTCGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACGGA CTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATC AGATACCGTCCTAGTCTTAACCATAAACCATGCCGACTAGAGATTGGAGGTCGTTATCTATACGACTCCT TCGGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAG GAATTGACGGAAGGGCACCACCAGGAGTGGAGCTTGCGGCTTAATTTGACTCAACACGGGGAAACTTACC AGGTCCAGACATAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCC GTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCCGCTAAATAGT TACATGTAACCTCGGTTACATGGGCAACTTCTTAGAGGGACTTTGCGTGTCTAACGCAAGGAAGTTTGAG GCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGTGCTACACTGACGCATTCAACGAC TTTACGACCTTGCCCGGAAGGGTTGGGTAATCTTCTGAAAATGCATCGTGATGGGGATAGATTATTGCAA TTATTAATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGTGCTGATTACGTCCCTGCCCT TTGTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGCGCCCA GCTCCTGGGTGCTGCAGTGGAAAGTTTGGTGAACCTTATCACTTAGAGGAAGGAGAAGTCGTAACAAGGT TTCC
Importance of organism and transcriptomes	The main growing stage of Thoracosphaera heimii is its encysted form, not the flagellated stage that is typical for dinoflagellates. The cyst of Thoracosphaera contains a calcium carbonate layer, and nothing is known about the genetic mechanisms that govern either the deposition of this substance in the cyst of the cell, or more importantly, the control of cell cycle life stages of dinoflagellates (flagellate cells vs. cysts, be they sexual or not). Taxonomically speaking Thoracosphaera is important because it is the only typical member of the Peridiniales that is included in our proposal (Heterocapsa is nominally also a peridinialean, but it is certainly not a typical one).
Additional citations and references	Meier, K.J.S., Young, J.R., Kirsch, M. & Feist-Burkhardt, S. (2007). Evolution of different life-cycle strategies in oceanic calcareous dinoflagellates. European Journal of Phycology 42: 81-89.
Primary citation for organism's characterization, if available	Inouye, I. & Pienaar, R.N. (1983). Observations on the life cycle and microanatomy of Thoracosphaera heimii (Dinophyceae) with special refeence to its systematic position. S.-Afr. Tydskr. Plantk. 2: 63-75.
Latitude	23.809
Longitude	-89.757
Collection date	23-APR-80
Sample collection site	Gulf of Mexico
Sample material (e.g. "seawater," "sediment," etc.)	seawater
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	02-JUN-11
Growth medium	HESNW
Modifications to growth medium	no silica
Temperature (ºC)	23
Salinty (psu)	30
pH	7
Light (µmol photons / m2 / sec)	3800
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	3
Ammonium (μmol/L)	.11
Phosphate (μmol/L)	.3
Investigation type	Eukaryotes