Polarella glacialis CCMP 1383

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Environmental Data
Principle Investigator(s)	Juan Saldarriaga
External sample ID	Polglac
NCGR Sample ID	MMETSP0227
Sample accession number	CAM_SMPL_002476
Assembly accession number	CAM_ASM_000294
Combined Assembly Name	N/A
Genus	Polarella
Species	glacialis
Strain	CCMP 1383
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	89957
18S rRNA	GGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCAGTATAAGCTTTTACACGGTGAAACTGCGAATGGCTCATTAAAGCAGTTATAGTTTATTTGATGGTCACTGTTACATGGATAACTGTGGTAATTCTAGAGCTAATACATGCACCAAAACCCAACTTCGCAGAAGGGTTGTGTTTATTAGATACAGAACCAACCCAGGCTCCGCCTGGTTTTGTGGTGGCTCATAATAACTAGATGAATCGTATGCTTCGGCGACGATGCATCATTCAAGTTTCTGACCTATCAGCTTCCGACGGTAGGGTATTGGCCTACCGTGGCAATGACGGGTAACGGAGAATTAGGGTTTGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGAATGAGTAGAATTTAATCCTTTATGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAGCTCGGTAGTTGGATTTCTGCCGAGGATGACCGGTCCGCCCTCTGGGTGAGTATCTGGCTCAGCCTTGGCATCTTCTTGAAGAACGTTACTGCACTTGACTGTGTGGTGCGGTACTCAGGACATTTACTTTGAGGAAATTAGAGTGTTTCAAGCAAGCGCACGCCTTGAATACATTAGCATGGAATAATAAGATAGGACCTCGGTTCTATTTTGTTGGTTTCTAGAGCTGAGGTAATGATTGATAGGGATAGTTGGGGGCATTCGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACGGACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCCTAGTCTTAACCATAAACTATGCCGACTAGAGATTGGAGGTCGTTATCTTTACGACTCCTTCAGCACCTTATGAGAAATCAAACTCTTTGGGTTCCGGGGGGAGTATCCTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACATGTAACCTCGGTTACATGGGCAACTTCTTAGAGGGACTTTGCGTGTCTAACGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCGCTCAACGAGTTACGATCTTGCCTGAAATGGCTGGGTAATCTTTTTAAAACGCATCGTGATGGGGATAGATCATTGCAATTATTGATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGTGCTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGCTTCTGGGCGTTGCGTTGGAAAGTTTAGTGAACCTTATCACTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC
Importance of organism and transcriptomes	Polarella glacialis is a common dinoflagellate of the order Suessiales that is found in marine high latitude waters, and as such its physiology is geared towards coping with low temperature and seasonally low light environments. Nothing is known about the genetic mechanisms that underlie those adaptations.
Additional citations and references	Thomson, P.G., Wright, S.W., Bolch, C.J.S., Nichols, P.D., Skerratt, J.H. & McMinn, A. (2004). Antarctic distribution, pigment and lipid composition, and molecular identification of the brine dinoflagellate Polarella glacialis (Dinophyceae). Journal of Phycology 40: 867-873
Primary citation for organism's characterization, if available	Montresor, M., Procaccini, G. & Stoecker, D.K. (1999). Polarella glacialis, gen. nov., sp. nov. (Dinophyceae): Suessiaceae are still alive!. Journal of Phycology 35: 186-197
Collection date	01-JUN-91
Sample collection site	McMurdo Sound
Sample material (e.g. "seawater," "sediment," etc.)	seawater
Habitat	marine habitat
Country	ANTARCTICA
Experimental Data
Date of experiment	18-JUL-11
Growth medium	HESNW
Modifications to growth medium	no silica
Temperature (ºC)	4
Salinty (psu)	30
pH	7
Light (µmol photons / m2 / sec)	3800
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	3
Ammonium (μmol/L)	.11
Phosphate (μmol/L)	.3
Investigation type	Eukaryotes