Protoceratium reticulatum CCCM 535 (=CCMP 1889)

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Environmental Data
Principle Investigator(s)	Juan Saldarriaga
External sample ID	Protret
NCGR Sample ID	MMETSP0228
Sample accession number	CAM_SMPL_002473
Assembly accession number	CAM_ASM_000291
Combined Assembly Name	N/A
Genus	Protoceratium
Species	reticulatum
Strain	CCCM 535 (=CCMP 1889)
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	133427
18S rRNA	AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCAGTATAATTTCAATATGGTTAAACTGCGAA TGGCTCATTAAAACAGTTATAGTTCAACTTGATGGTGTGTGTTACATGGATAACTGTGGTAATTCTAGAG CTAATACATGCTTCTTGGCCCGACTTTCGCGGAAGGGCTGTTTTTATTAGTTACAAAACTAACCTAGACT CTGTCTAGTCTTTTGTTGATTCATGATGTTTGGATGGATTGCATGGCAGCAGCTGGTGACACATCATTCA AGTTTCTGACCTATCAGCTTCCGACGGTAAGGTATTGGCATACCGTGGCAATAACGGGTAACGGAGAATT AGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAAT TACCCAATCTTGACACAAGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGA ATGAATAGAATTCAAACCCTTTTGTTAGTATCGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAAT TCCAGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGCTGAAGACA ACCGGTCTGCCCTCTGGGTGAGCATCTGGTTTGGATTCGGCGTCATCTTAGAGAACGTATCTGCACTTGA CTGTGTGGTGCGGTATCTTAGACTTTTACTTTGAGGAAATCAGAGTGCTTCAAGCAGGCGAATGCCTTGG ATACATTAGCATGGAATAATATTTTAGGACCTTGGTTCTATTTTGTTGGTTTCTAGAATTGAGGTAACGT TTAATAGGGATAATTGGGGGCATTCGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACGG ACCAATGCGAAAGCATTTGCCAAGGATGTTTTCTTTGATCAAGAACGAAAGTTAGGGGATCGAAGACGAT CAGATACCGTCCTAGTCTTAACCATAAACCATGCCGACTAGAGATTGGAGGTCGTTATTTGTACGACCCC TTCGGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAA GGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTAC CAGGTCCAGACATAGCAAGGATTGACAGATTGATAGCTCTTTCTTGATTTTATGGGTGGTGGTGCATGGC CGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACTTGCTAAATAG TTACGTGTAATTTTGGTTACATGGGCAACTTCTTAGAAGGACTTTGTGTGTCTAACGCAAGGAAGTTTGA GGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATGCGGTCAACAA GTTTTTGACCTGGCCTGGAAAGGTTGGGTAATCTATTGAACACGCATCGTGATGGGGATAGCTTATTGCA ATTATTAATCTTCAACGAGGAATTCCTAGTAAGCGTGAGTCATCAGCTCATGCTGATTACGTCCCTGCCC TTTGTACGCACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTTGGACTGTGGCAATGTCT AGCTCTAGACTTTGCTGTAGAAAATCTTATGAACCTTATCACTTAGAGGAAGGAGAAGTCGTAACAAGGT TTCT
Importance of organism and transcriptomes	Protoceratium reticulatum is a `core? member of the order Gonyaulacales and is a heavily armoured dinoflagellate that has been shown to contain yessotoxin, a substance toxic to mice but whose effects on humans are not known. It is also a common member of the summer plankton in temperate latitudes.
Additional citations and references
Primary citation for organism's characterization, if available	Satake M., MacKenzie L. & Yasumoto T. 1997. Identification of Protoceratium reticulatum as the biogenetic origin of yessotoxin. Nat. Toxins 5: 164-167.
Sample material (e.g. "seawater," "sediment," etc.)	seawater
Habitat	marine habitat
Experimental Data
Date of experiment	02-JUN-11
Growth medium	HESNW
Modifications to growth medium	no silica
Temperature (ºC)	18
Salinty (psu)	30
pH	7
Light (µmol photons / m2 / sec)	3800
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	3
Ammonium (μmol/L)	.11
Phosphate (μmol/L)	.3
Investigation type	Eukaryotes