Pyrocystis lunula CCCM 517

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Environmental Data
Principle Investigator(s)	Juan Saldarriaga
External sample ID	Pyrlun
NCGR Sample ID	MMETSP0229_2
Sample accession number	CAM_SMPL_002474
Assembly accession number	CAM_ASM_000292
Combined Assembly Name	N/A
Genus	Pyrocystis
Species	lunula
Strain	CCCM 517
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	2972
18S rRNA	AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTTTAAACCTTTCATGGTGAAGCTGCAAA TGGCTCATTAAAACAGTTATAATTCAAATGTTGGTTGCAATTTACATGGATAACCTCGGTAATTCTGCAG CTAATTCATGCCATCAAACTTGACTTGTTGAAGATAAAGTTGTGGTTTTTAGCTTTTAGAATCAACCCAA GCTCTGCTTGGTTTTGTGGTGATGCATAAGGACTCAATGAATTGCATAGCATACGCTGGCGATGCAACAT TCAAGTTTCTGACCTATCAGCTTCCGACGGTAGGGTATTGGCTTACCGTGGCATTGACGGGTAACGGGGG ATTAGGGTTTGATTCCGGAGAGGGAGCCTGGGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCA AATTACCCAATCCTGACACAGGGAGGTAGTGACAAGATATAACAATACAAGGCATCCATGTTTTGTAATT GGAATGAATGGAATTTAAATCACTTTCTGAGTATCGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT AATTCCAGCTCCAAAAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGACTTCTGCTGGGG CTGGCTGGTCTACCCGCTGGGTGAGTATCTGGCATAGCCTGAGCATCTGTTTGAAATATGCTACTGCACT TGACTGTGTGGTGTGGTATTTATCGCTTTTACTTTGAGAAAATCAGAGTGTTCCACGCAGGCGCATGCCT TGTATATGTGAGCATGGAATAATGTTTCAGGGCTTTGGTTTTTTGTTGGTTTTTAAAACCAGGGTAATGG TGAATAGGGATAGTTGGGGGCACTTGTATTTAATTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACAA ACTACTGCGAAAGCATTTGCCAAAGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGAT CAGATACCGTCCTAGTCTTAACCATAAACTATGCCGACTAGAGATTGGATGCCGTTATCCGTAGGATGCC TTCAGCACCCTGTGAAAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAA GGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTAC CAGGCCCGGACATAATTAGGATTGACAGATTGATAGCTTTTTCTTGATTCTATGGGTGGTGGTGCATGGC CGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAG TTACATGTAATTTCGGTTATGTGGGCAACTTCTTAGAGGGACTTCGTGTGTCTAACATGAGGAAGTTTGA GGCGATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGTGCTACACTGACGCACTCAACAG GTTTTTCACCTCGCCTGGAAGGGTTGGGTAATCTTCAACATGTATCGTGATGGGGATAGATTGTTGCAAT TATTAATCTTGAACGAGGAATTCCTAGTAAGCCGAAGTCATCAGCTTGGGCTGATTACGTCCCTGCCCTT TGTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATTACTTGGATTGCAGCAGAGCTCAG TGTGTGAGCCTTGCTGAAGAAATGTCATGAACCTTATTACTTAGAGGAAGGAGAAGTCGTAACAAGG
Importance of organism and transcriptomes	Pyrocystis lunula is interesting for two main reasons. First, it is one of very few dinoflagellates that spend the majority of their time as metabolically active cysts and not in their flagellated stage. Second, it is also an intensely bioluminescent species that has served as a model system to study this process as well as circadian rhythms.
Additional citations and references	Swift, E. & Taylor, W.R. (1967). Bioluminescence and chloroplast movement in the dinoflagellate Pyrocystis lunula. Journal of Phycology 3: 77-81.
Primary citation for organism's characterization, if available	Heimann, K., Klerks, P.L. & Hasenstein, K.H. (2009). Involvement of actin and microtubules in regulation of bioluminescence and translocation of chloroplasts in the dinoflagellate Pyrocystis lunula. Botanica Marina 52(2): 170-177
Sample material (e.g. "seawater," "sediment," etc.)	seawater
Habitat	marine habitat
Experimental Data
Date of experiment	02-JUN-11
Growth medium	HESNW
Modifications to growth medium	no silica
Temperature (ºC)	23
Salinty (psu)	30
pH	7
Light (µmol photons / m2 / sec)	3800
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	3
Ammonium (μmol/L)	.11
Phosphate (μmol/L)	.3
Investigation type	Eukaryotes