Debaryomyces hansenii J26

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Environmental Data
Principle Investigator(s)	Nadathur Govind
External sample ID	0.5 h exposure to cobalt(II)
NCGR Sample ID	MMETSP0233
Sample accession number	CAM_SMPL_002492
Assembly accession number	CAM_ASM_000310
Combined Assembly Name	Debaryomyces-hansenii-J26
Genus	Debaryomyces
Species	hansenii
Strain	J26
Clonal	Yes
Axenic	Yes
Prelim. NCBI Taxon ID	284592
18S rRNA	CTGGGTTGGATCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTACCTTTACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATCCCGACTGTTTGGAAGGGATGTATTTATTAGATAAAAAATCAATGCTTTTCGGAGCTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTAGTGACAATAAATAACGATACAGGGCCCTTTCGGGTCTTGTAATTGGAATGAGTACAATGTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGCTTGGTTGGCCGGTCCGCCTTTTTGGCGAGTACTGGACCCAACCGAGCCTTTCCTTCTGGCTAACCTTTCGCCCTTGTGGTGTTTGGCGAACCAGGACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCTTTGCTCGAATATATTAGCATGGAATAATAGAATAGGACGTTATGGTTCTATTTTGTTGGTTTCTAGGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAGTTGTCAGAGGTGAAATTCTTGGATTTACTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGGTGTTGTTCTTTTTTTGACGCACTCGGCACCTTACGAGAAATCAAAGTCTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAATAGTGCTGCTAGCTTTTGCTGGTATAGTCACTTCTTAGAGGGACTATCGATTTCAAGTCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAACGAGTATTAACCTTGGCCGAGAGGTCTGGGAAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGTTTAAAGAAGGGGGCAACTCCATCTTGGAACCAAAAAGCTGGTCAAACTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAGGGTTTCCGTAGGTGAACCCCTGCGGA
Importance of organism and transcriptomes	The purpose of this study is to analyze the molecular response of D. hansenii in the presence of a bioavailable toxic metal so as to better understand its effect on biological processes within the organism and its ability to tolerate metal stress. This transcriptomic analysis of differential gene expression will help us to evaluate prospective reporter genes that may or may not be directly associated to metabolic pathways related to metal tolerance as a first step in the development of a potential biosensor for heavy metal-contaminated marine areas. This transcriptome will show gene expression levels in D. hansenii after exposure to cobalt(II).
Additional citations and references	19235772
Primary citation for organism's characterization, if available	Norkrans (1966). Studies on marine occurring yeasts: Growth related to pH, NaCl concentration and temperature. Arch. Microbiol. 54(4):374-392
Experimental Data
Date of experiment	14-JUL-11
Growth medium	Yeast-Peptone-Dextrose (2% each) Broth
Modifications to growth medium	5 mmol/L CoSO4*7H2O added at early log phase
Temperature (ºC)	25
pH	6.5
Investigation type	Eukaryotes