Prorocentrum lima CCMP 684

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Environmental Data
Principle Investigator(s)	Juan Saldarriaga
External sample ID	Prorlima
NCGR Sample ID	MMETSP0252
Sample accession number	CAM_SMPL_002496
Assembly accession number	CAM_ASM_000314
Combined Assembly Name	N/A
Genus	Prorocentrum
Species	lima
Strain	CCMP 684
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	39448
18S rRNA	TTGTCTCAAAGATTAAGCCATGCATGTCTCAGCATAAGCTTCCATCCGGCGAAACTGCGAATGGCTCATTAAAACAGTTACAATTTATTTGGTGGTTCACTGTTACATGGATACCCGTGGAAATGCTAGAGCTAATACATGCGCTCCTACCCGACTTAGCAGAAGGGTTGTGGTTATTAGTTACAGAACTAGCCCAGGCTTGCCTGGTCATGCGGTGACTCATGACAATGGAATTAGTCGTATGGCGTCTGCTGACGATAAATCATTCAAGCTTCTGACCTATCAGCTTCCGACGGTAGGGTATTGGCCTACCGCGGCTTTGACGGGTAACGGAGAATTAGGGTTTGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGAATGAGTAGAACTTAAATCTCTTTATGAGTACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCATATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTCGGATTTCTGCCGAGGACGACCGGTCCGCCCTCTGGGTGAGCATCTGGCTTGATCTGGGCATCTTCTTGGAGAGCGTAGCTGCACTTGACTGTGTGGTGCGGTATCCAGGACTTTTACTTTGAGGAAATTAGAGTGTCCTAAGCAGGCCCATGCCATATATACATTAGCATGGAATAATAGGGTAGGACCTACTCTCTATTTTGTTGGTTTCTAGAGCAGAGGTAATGGTCAATGGGGATAGTTGGGGGTATCCGTATTTGACTGTCAGAGGTGAAATTCTTGGATTTGTCAAAGACGAACCAATGCGAAAGCATTTGCCAGAGATGTTTTCCTTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCCTAGTCTTAACCATAAACTATGCCAACTAGAGATTGGAGGTCGTTATGTTGACGACTCTTTCGGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCGGACATAGTAAGGATTGACAGATTGACAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACTTGCTGAATAGCTACTTCTAACCCCGGTTACATGGGCAGCTTCTTAGAGGGACTTTGCTGTGTCTAACGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATGCGCCCAATGAGTTTTTGACCTTGCCTGGTAAGGTTGGGTAATCTGTCAAAAAACGCATCGTGATGGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAAGTATGAGTCATCAATTCGTGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTCAGACTCTGCAGCTCTCAACTCGCGAGTGTTGCAATGGAAAGTTTAGTGAGCCTTATCACTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAG
Importance of organism and transcriptomes	Prorocentrum lima is a common benthic dinoflagellate that produces a number of toxic substances including okadaic acid, the toxin resposible for diarrheic shellfish poisoning. Its morphology is very derived, and nothing is known about the genetic features that underline that. In SSU-based phylogenetic trees the genus Prorocentrum tends to split into two groups, and P. lima does not cluster with the type species of the genus, P. micans.
Additional citations and references	Barbier, M., Amzil, Z., Mondeguer, F., Bhaud, Y., Soyer-Gobillard, M.-O. & Lassus, P. (1999). Okadaic acid and PP2A cellular immunolocalization in Prorocentrum lima (Dinophyceae). Phycologia 38: 41-46
Primary citation for organism's characterization, if available	Grzebyk, D., Sako, Y. and Berland, B. 1998: Phylogenetic analysis of nine species of Prorocentrum (Dinophyceae) inferred from 18S ribosomal DNA sequences, morphological comparisons and description of Prorocentrum panamensis sp. nov. J. Phycol. 34: 1055-1068
Collection date	01-JUN-91
Sample collection site	Ria de Vigo, Galicia
Sample material (e.g. "seawater," "sediment," etc.)	seawater
Habitat	marine habitat
Country	SPAIN
Experimental Data
Date of experiment	18-JUL-11
Growth medium	HESNW
Modifications to growth medium	no silica
Temperature (ºC)	22
Salinty (psu)	30
pH	7
Light (µmol photons / m2 / sec)	3800
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	3
Ammonium (μmol/L)	.11
Phosphate (μmol/L)	.3
Investigation type	Eukaryotes