Noctiluca scintillans Unknown

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Environmental Data
Principle Investigator(s)	Juan Saldarriaga
External sample ID	Noctscin
NCGR Sample ID	MMETSP0253
Sample accession number	CAM_SMPL_002497
Assembly accession number	CAM_ASM_000315
Combined Assembly Name	N/A
Genus	Noctiluca
Species	scintillans
Strain	Unknown
Clonal	No
Axenic	No
Prelim. NCBI Taxon ID	2966
18S rRNA	CACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCAGTATCAAC TTTTACACGGTAACACTGCGAATGGCTCATTAAAACAGTTATAGTTTATTTGATGGCATTCATTATATGG ATACCTTTGGTAATTCTAGAGCTAATACATGCATACAGACCCGACTCATGAAGGGTTGTGTTTATTAGAT TCCAAACCTTCCAACTTCGGTTGTGTTCTTGGTGATTCATGATAAAATACTGATCGCCTTTGGCGACGAG TCTTTCAAGTTTCTGACCTATCAGCTTTCGATGGTAGGGTATTGGCCTACCATGGCAGTGACGGGTAACA GAGAATTAGGGTTCGATTCTGGAGAGGGAGCCTGAGAAACGGCTACCACTTCTAAGGAAGGCAGCAGGCG CGTAAATTACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGT AATTGGAATGAGTAAAATTTAAATCTCTCTACGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCG CGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGCC GAGGACAGTCGGTCCGCCCTTGCGGGTGAGCATCTGAATGGCCCTGGGCATCTTTTCCGAAGACTGTATC TGCACTTCATTGTGTGGAGCGGTATTCGGAACCTTTACTTTGAGGAAATTAGAGTGTTTCAGGCATGCGC TTGTGGTGAATACATTAGCATGGAATAATAAGATAGGACTCGTGGCCTACTTTGTTGGTTTGTGTGCCAT AGGTAATGATTAATAGGGATAGTTGGGGGCATTCGTATTTAATTGTCAGAGGTGAAATTCTTGGATTTGT TAAAGACGAACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATC GAAGACGATCAGATACCGTCGTAGTCTTAACCATAAACCATGCCGACTAGAGATTGGGGGTCGTTAGCTT TATGACTCCTTCAGCACCTTATGAGAAATCAAAGTTTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTG AAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGG AAAACTCACCAGGTCCAGACATAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTG GTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAGCCT GCTAAATAGTGTCAAGTATGATTCTTATTTGATTACTTCTTAGAGGGACTTTGTGTGTCTAACGCAAGGA AGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATGCAT TCAACGAGTTTATAACCTTGCCTGAAAAGGTTGGGTAATCTGCAATGTGCATCGTGATGGGGATAGATTA TTGCAATTATTAATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGTGCTGATTACGTCCC TGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAGTGATTCGGTGAATAATTCGGAGATTGATTT GTTCAGCTTTGCAGAACATGTCTTTCGAAGTCTAGTGAACCTCATCACTTAGAGGAAGGAGAAGTCGTAA CAAGGTTTCCGTAGGTGAACCTGCAGAAGGATCAAGC
Importance of organism and transcriptomes	Noctiluca scintillans is a common, bloom-forming voracious predator in the plankton of all oceans. It is a very atypical dinoflagellate, and much of its biology, especially in regards to the organization of its nucleus is not understood. Its vegetative life stage is up to 2 mm in diameter, and much of its volume is a vacuolar space. Whether or not its exceedingly large nucleus is dinokaryotic in this stage has not been determined, but it is capable of making flagellated stages, probably gametes, that do have the typical dinoflagellate morphology and a typical dinoflagellate nucleus. Noctiluca is otherwise interesting for several reasons. First, it is capable of producing spectacular orange blooms that, although not toxic in themselves, have caused problems by producing large quantities of ammonia, blocking sunlight and depleting oxygen. Second, it is one of the most bioluminescent of dinoflagellates. Third, some strains are capable of harbouring green prasinophytes within their vacuolar system and become practically autotrophic; very little is known about how this endosymbiosis works. And fourth, the phylogenetic position of the whole order is contested but may be pivotal to understanding the evolution of many strange dinoflagellate characters because it may be an early-branch.
Additional citations and references	Gomez, F., Moreira, D. and Lopez-Garcia, P. 2010: Molecular phylogeny of noctilucoid dinoflagellates. Protist 161: 466-478
Primary citation for organism's characterization, if available	Hoppenrath, M. and Leander, B. 2010: Dinoflagellate phylogeny as inferred from heat shock protein 90 and ribosomal gene sequences. PLoS One 5: e13220
Collection date	12-APR-94
Sample collection site	Puget Sound, WA
Sample material (e.g. "seawater," "sediment," etc.)	seawater
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	18-JUL-11
Growth medium	HESNW
Modifications to growth medium	no silica
Temperature (ºC)	16
Salinty (psu)	30
pH	7
Light (µmol photons / m2 / sec)	3800
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	3
Ammonium (μmol/L)	.11
Phosphate (μmol/L)	.3
Investigation type	Eukaryotes