Amphidinium carterae CCMP1314

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Environmental Data
Principle Investigator(s)	Geoff Sinclair, Boris Wawrik
External sample ID	A. carterae L1
NCGR Sample ID	MMETSP0258
Sample accession number	CAM_SMPL_002498
Assembly accession number	CAM_ASM_000316
Combined Assembly Name	Amphidinium-carterae-CCMP1314
Genus	Amphidinium
Species	carterae
Strain	CCMP1314
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	2961
18S rRNA	CTTCATCGCACATGGATAACTGTGGAAAATCTAGGGCTAATACATGCGTCATACCTGACTTCTGGGAAAGGTTGCTATCA TGAGTCATAGAATCGGCGCAGGCTCTGCCTTGCTACCAAGGTGAGTCATGATAATTTAGCTGATCGCATTGCATCAGCTG GTGATGAACTGCACAAGTTTCTGACCTATCAGCTTCCGACGCTAGGGTATTGGCCTACTGTGGCAATGACGGGTGACGGA GAATTAGGGTTCGATTCCGGAGAGGGAGCCTGACACATGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCC AATCCTGACATGGGGAGGTAGTGACAAGCAATAACAATACGGGGCATCCATGTCTTGTAATTGGAATGAGCTAACTGTAA ACATGTTGGTGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA GCTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGTCaAGTACAACTGGTCCGCCCTCTGGGTGAGCACATAGTTTGGT CTTGGCATCTTGCTGGAAACTGTTGCTGCACTTGGCTGTGTGGCGCAGTGTCCAGCACTTTCACTTTGAGGAAATTGGAG TGTTTCAAGCAGGCAATGCCTTTGAACACAGTAGCATGGAATAATTAGATTGGGCTTTTGCTCCAATTTGTCGGTTTCTG GAGCTGAGGTGAGAATTAATGGGGATTGTTGGGGGCATCCGAATTCAGCCGTCAGAGGTGAAATTCTTGGATCGGCTAAA GACGGACAACTGCGAAAGCGTTTGCCCAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGAT ACCGTCCTAGTCTTAACCATAAACTATGCCAACTAGGGATTGGTGGTCGTTAGTTGCACGACCCCATCAGCACCTGATGA GAAATCAAAGTCTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAG GAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGGTAGGATCGACAGATTGATA GCTCTTTCGTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACG AACGAGACCTTATCTGGCTAAGTAGCTATGCATAGCTGTGGTTTTGTGGACAGTTTCTTAGAGGGACTTCGTGCGCCTAG CATGAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTYTGGGCTGCACGCGCGCTACACTGATACCTTC AGCGAGTCCACCACCTTGCCTGGGAGGGTGGGGTAACCTCTGGAAAGTGCATCGTGATGGGGATCGATTATTGTAATTAT TAATCTTCAACGAGGAATTCCTAGTAAGCATGTGTCATCAGCCCGTGCTGATTACGTCCCTGCCCTTTGTACACACCGCC CGTCGCTCCTACCGATTGAGTGATCCGGTGAATTA
Importance of organism and transcriptomes	Dinoflagellates are a key component of the eukaryotic phytoplankton in all of the world?s oceans. Despite their importance, mechanistic understanding of their physiological responses to important environmental changes, such as nutrient loading remains relatively poorly understood, since very little genetic information is available due to the immense size of their genomes. Transcriptome sequencing will provide significant insight into the genomic complexity of this group and a foundation upon which to explore the linkages between genomic and phenotypic responses in response to environmental changes that threaten coastal ecosystems.
Additional citations and references
Latitude	41.56
Longitude	70.583
Habitat description	coastal
Habitat	marine habitat
Experimental Data
Date of experiment	01-AUG-11
Growth medium	L1
Modifications to growth medium	none
Temperature (ºC)	22
Salinty (psu)	32
pH	8.2
Light (µmol photons / m2 / sec)	60
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	60
Phosphate (μmol/L)	60
Trace elements (total) (nmol/L)	1170
Prey organism, if applicable (genus and species)	none
Other experimental metadata available	Isolated from Falmouth Great Pond, Falmouth, Massachusetts USA