Prorocentrum minimum CCMP2233

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Environmental Data
Principle Investigator(s)	Geoff Sinclair, Boris Wawrik
External sample ID	P.minimum L1
NCGR Sample ID	MMETSP0267
Sample accession number	CAM_SMPL_002486
Assembly accession number	CAM_ASM_000304
Combined Assembly Name	Prorocentrum-minimum-CCMP2233
Genus	Prorocentrum
Species	minimum
Strain	CCMP2233
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	39449
18S rRNA	GGGTGTACAAAGGGCAGGGACGTAATCAGCACGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTGAAGATTAATAA TTGCAATAATCTATCCCCATCACGATGCGATTTAAAAAGATTACCCAACCTTTCCAGGCAAGGTCATAAACTCGTTGAAC GCATCAGTGTAGCGCGCGTGCAGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGCGTTAG ACACGCAAAGTCCCTCTAAGAAGTTGCCCACGTAACCGAAGTTACGTGTAACTATTTAGCAGGTTAAGGTCTCGTTCGTT AACGGAATTAACCAGACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTATC AATCTGTCAATCCTTACTATGTCTGGACCTGGTAAGTTTCCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGG TGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT AAGGTGCTGAAGGAGTCGTATAAATAACGACCTCCAATCTCTAGTCGGCATGGTTTATGGTTAAGACTAGGACGGTATCT GATCGTCTTCGATCCCCTAACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTCCGTCTTTA ACAAATCCAAGAATTTCACCTCTGACAGTTAAATACGAATGCCCCCAACTATCCCTATTAATCATTACCTCAGCTCTAGA AACCAACAAAATAGAACCGAGGTCCTATCTTATTATTCCATGCTAATGTATTCAAGGCGTAAGCCTGCTTGAAACACTCT AATTTCCTCAAAGTAAAAGTCCTGGATACCGCACCACACAGTCAAGTGCAGCTACGTTCTCCAAGAAGATGCCCAGGCCG AgCCAGATACTCACCCAGAGGGCGGACCGGTCGTCCTCGGCAGAAATCCAACTACGAGCTTTttAACCGCAACAACTTTA ATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTACTCGTAAAGGGATTTAA ATTCTACTCATTCCAATTACAAGACAATTATGCCCTGTATTGTTATTTCTTGTCACTACCTCCCTGTGTCAGGATTGGGT AATTTGCGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCG TTACCCGTCATTGCCACGGTAGGCCAATACCCTACCGTCGGAAGCTGATAGGTCAGAAACTTGAATGATTCATCGCCAGC GAAGCTATGCGATTCGTTCGGTTATTATGAATCACCAAAGGACCAGGCGGAGCCTGGGCTGGTTCTGTAACTAATAAACA CAACCCTTCCTATAAGTCGGGTTTGGGCGCATGTATTAGCTCTACAATTGGCACAGTTATCCATGTAAAGAATGACCATC AA
Importance of organism and transcriptomes	Dinoflagellates are a key component of the eukaryotic phytoplankton in all of the world?s oceans. Despite their importance, mechanistic understanding of their physiological responses to important environmental changes, such as nutrient loading remains relatively poorly understood, since very little genetic information is available due to the immense size of their genomes. Transcriptome sequencing will provide significant insight into the genomic complexity of this group and a foundation upon which to explore the linkages between genomic and phenotypic responses in response to environmental changes that threaten coastal ecosystems.
Additional citations and references
Latitude	38.59
Longitude	75.1
Habitat description	coastal
Habitat	marine habitat
Experimental Data
Date of experiment	01-JUL-07
Growth medium	L1
Modifications to growth medium	none
Temperature (ºC)	22
Salinty (psu)	34
pH	8.2
Light (µmol photons / m2 / sec)	60
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	60
Phosphate (μmol/L)	60
Trace elements (total) (nmol/L)	1170
Prey organism, if applicable (genus and species)	none
Other experimental metadata available	Isolated from Indian River Bay, Delaware USA (IR32)