Scrippsiella trochoidea CCMP3099

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Environmental Data
Principle Investigator(s)	Geoff Sinclair, Boris Wawrik
External sample ID	S. trochoidea L1
NCGR Sample ID	MMETSP0270
Sample accession number	CAM_SMPL_002487
Assembly accession number	CAM_ASM_000305
Combined Assembly Name	Scrippsiella-trochoidea-CCMP3099
Genus	Scrippsiella
Species	trochoidea
Strain	CCMP3099
Clonal	No
Axenic	No
Prelim. NCBI Taxon ID	71861
18S rRNA	TATTTGATGGTCATTCCTTACATGGATAACTGTGGTAATTCTAGAGCTAATACATGCGCCCAAACCCGACTCCGTGGAAG GGTTGTGTTTATTAGTTACAGAACCAACCCAGGCTCTGCCTGGTCTTGTGGTGATTCATAATAACCGAATGAATCGCATT GCATCAGCTGGCGATGAATCATTCAAGTTTCTGACCTATCAGCTTCCGACGGTAGTGTATTGGACTACCGTGGCAATGAC GGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCG CAAATTACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGAATGAG TAGAATTTAAATCCCTTTATGAGTATCGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGC GTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGCTGAGGACGACCGGTCCGCCCTCTGGGTGAGTAT CTGGCTCGGCCTGGGCATCTTCTTGGAGAACGTAGCTGCACTTGACTGTGTGGTGCGGTATCCAGGACTTTTACTTTGAG GAAATTAGAGTGTTTCAAGCAGGCACACGCCTTGAATACATTAGCATGGAATAATAAGATAGGACCTTGGTTCTATTTTG TTGGTTTCTAGAGCTGAGGTAATGATTAATAGGGATAGTTGGGGGCATTCGTATTTAACTGTCAGAGGTGAAATTCTTGG ATTTGTTAAAGACGGACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAG ACGATCAGATACCGTCCTAGTCTTAACCATAAACCATGCCGACTAGAGATTGGAGGTCGTTATTTGTATGACTCCTTCAG CACCTTATGAGAAATCaAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAG GGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTG ACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAA TTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACACGTAACCTCGGTTACGTGGGCAACTTCTTAGAGGGACTT TGCGTGTCTAACGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACA CTGATGCGCTCAACGAGTTTATGACCTTGCCCGGCAGGGTTGGGTAATCTTCTTAAAACGCATCGTGATGGGGATAGATT ATTGCAATTATTAATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGTGCTGATTACGTCCCTGCCCTTTG TACACACCGC
Importance of organism and transcriptomes	Dinoflagellates are a key component of the eukaryotic phytoplankton in all of the world?s oceans. Despite their importance, mechanistic understanding of their physiological responses to important environmental changes, such as nutrient loading remains relatively poorly understood, since very little genetic information is available due to the immense size of their genomes. Transcriptome sequencing will provide significant insight into the genomic complexity of this group and a foundation upon which to explore the linkages between genomic and phenotypic responses in response to environmental changes that threaten coastal ecosystems.
Additional citations and references
Latitude	27.3122
Longitude	82.601
Habitat description	coastal
Habitat	marine habitat
Experimental Data
Date of experiment	01-AUG-11
Growth medium	L1
Modifications to growth medium	none
Temperature (ºC)	22
Salinty (psu)	32
pH	8.2
Light (µmol photons / m2 / sec)	60
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	60
Phosphate (μmol/L)	60
Trace elements (total) (nmol/L)	1170
Prey organism, if applicable (genus and species)	none
Other experimental metadata available	Isolated from off St. Armands Key, Florida, USA