Skeletonema marinoi SM1012Hels-07

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Environmental Data
Principle Investigator(s)	Satoshi Nagai
External sample ID	SM1012Hels-07
NCGR Sample ID	MMETSP0319
Sample accession number	CAM_SMPL_002507
Assembly accession number	CAM_ASM_000325
Combined Assembly Name	N/A
Genus	Skeletonema
Species	marinoi
Strain	SM1012Hels-07
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	267567
18S rRNA	AACCTGGTTGATCCTGCCAGTAGTCATACGCTCGTCTCAAAGATTAAGCCATGCATGTGTAAGTATAAGATACTTTATCGAAACTGCGGACGGCTCATTATATCAGTTATAGTTTATTTGATAATCCCTCACTACTTGGATAACCGTAGTAATTCTAGAGCTAATATATGCATCAAAGCGGAACTCTCGGGAACCGCCGTGTTTATTAGTATTAAACCTTCACTCTTCGGAGTTGATTTGGTGAGTCATAATAACCTTTCGAATTGCATGACCACGTGTCGGCAATGGATCATTCAAGTTTCTGCCCTATCAGCTTGGGATGGTAGTGTATTGGACTACCATGGCTTTAACGGGTAACGAATTGTTAGGGCAAGATTTCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATGCCGGGCCTTTACAGGTCTGGCAATTGGAATGAGAACAATTTAAATCCCTTATCGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGCAGGAGTGACTGACCACAAACTCTGTTTGTGAGTTGTGTCATTCTGGCCATCCTTGGTAAGATCCTTTTTGGCATTAAGTTGTCGGGAAGGGGATGACCATCGTTTACTGTGAAAAAATTAGAGTGTTTAAAGCAGGCTTATGCCGTTGAATATATTAGCATGGAATAATAAGATAGGACTTTGAGTCTATTTTGTTGGTTTGCGAGTCAAAGTAATGATTAATAGGGACAGTTGGGGGTATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTCTGAAAGACGAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACTCAGGATTGGCGGTTGTTTTTTGACTCCGTCAGCACTGTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAACCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGTTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCGCCGCCTGCTAAATAGACCCGCGAATGGCTTTTCATTGGCGAGGTCTTCTTAGAGGGACGTTCATTCTACAAGATGAAGGAAGATGGCGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCCGCACGCGCGTTACACTGATGCACTCAACGAGTATATAACCTTGGCCGAGAGGCCTGGGTAATCTTGTTAACTTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAATCGCAGTTCATCAAACTGCAATGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGTCTAGCTCCTTTATTGGGGCTTGACCGCGAGAACCTGTCTAAACCTTATCATTTAGAGGAAGGTGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCA
Importance of organism and transcriptomes	This species has adapted to low water temperatures at 2-4 degrees Celsius in Baltic Sea and also relatively high water temperature at 20-25 degrees Celsius in coastal waters in Denmark or others. By comparison with the transcriptome data between them, we will be able to know the difference of gene expression associated with temperature responses. I have some more candidates which are able to compare the temperature responses and would like to conduct this in near future.
Additional citations and references
Latitude	59.51
Longitude	23.15
Depth (m)	5
Salinity (psu)	6
Temperature (ºC)	4
pH	7.8
Collection date	30-NOV-11
Collection time	01:00 PM
Sample collection site	Baltic Sea
Sample material (e.g. "seawater," "sediment," etc.)	sediment
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Other
Habitat description	eutrophicated lower salinity layer
Other environmental metadata available	no other types of environmental metadata available
Other environmental metadata available	no other types of environmental metadata available
Habitat	marine habitat
Country	FINLAND
Experimental Data
Date of experiment	25-MAR-11
Growth medium	modified f/2 amended seawater
Modifications to growth medium	f/2 for all nutrients without copper and silicate, but with H2SeO3 to be 10 (nmol/L)
Temperature (ºC)	4
Salinty (psu)	15
pH	8.5
Light (µmol photons / m2 / sec)	150
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	880
Phosphate (μmol/L)	36
Total Fe (nmol/L)	4
Trace elements (total) (nmol/L)	355
Investigation type	Eukaryotes