Marine Microbial Eukaryote Transcriptome Sequencing Project
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Skeletonema marinoi SM1012Den-03
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| Principle Investigator(s) | Satoshi Nagai |
|---|---|
| External sample ID | SM1012Den-03 |
| NCGR Sample ID | MMETSP0320 |
| Sample accession number | CAM_SMPL_002504 |
| Assembly accession number | CAM_ASM_000322 |
| Combined Assembly Name | N/A |
| Genus | Skeletonema |
| Species | marinoi |
| Strain | SM1012Den-03 |
| Clonal | Yes |
| Axenic | No |
| Prelim. NCBI Taxon ID | 267567 |
| 18S rRNA | |
| Importance of organism and transcriptomes | This species has adapted to low water temperatures at 2-4 degrees Celsius in Baltic Sea and also relatively high water temperature at 20-25 degrees Celsius in coastal waters in Denmark or others. By comparison with the transcriptome data between them, we will be able to know the difference of gene expression associated with temperature responses. I have some more candidates which are able to compare the temperature responses and would like to conduct this in near future. |
| Additional citations and references | |
| Environmental Data | |
| Latitude | 55.5837 |
| Longitude | 12.412 |
| Depth (m) | 20 |
| Salinity (psu) | 30 |
| Temperature (ºC) | 24 |
| pH | 7.8 |
| Collection date | 01-SEP-11 |
| Collection time | 03:00 PM |
| Sample collection site | North Sea |
| Other collection site info | Oresund |
| Sample material (e.g. "seawater," "sediment," etc.) | sediment |
| ENVO term for habitat - primary term | Acquatic: marine |
| ENVO term for habitat - secondary term | Other |
| Habitat description | eutrophicated lower salinity layer |
| Other environmental metadata available | no other types of environmental metadata available |
| Other environmental metadata available | no other types of environmental metadata available |
| Habitat | marine habitat |
| Country | DENMARK |
| Experimental Data | |
| Date of experiment | 25-MAR-11 |
| Growth medium | modified f/2 amended seawater |
| Modifications to growth medium | f/2 for all nutrients without copper and silicate, but with H2SeO3 to be 10 (nmol/L) |
| Temperature (ºC) | 25 |
| Salinty (psu) | 15 |
| pH | 8.5 |
| Light (µmol photons / m2 / sec) | 150 |
| Day portion of day:night cycle in hours | 12 |
| Night portion of day:night cycle in hours | 12 |
| Nitrate (μmol/L) | 880 |
| Phosphate (μmol/L) | 36 |
| Total Fe (nmol/L) | 4 |
| Trace elements (total) (nmol/L) | 355 |
| Investigation type | Eukaryotes |