Marine Microbial Eukaryote Transcriptome Sequencing Project
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Leptocylindrus danicus var. danicus B650
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| Principle Investigator(s) | ADRIANA ZINGONE |
|---|---|
| External sample ID | B650 |
| NCGR Sample ID | MMETSP0321 |
| Sample accession number | CAM_SMPL_002505 |
| Assembly accession number | CAM_ASM_000323 |
| Combined Assembly Name | N/A |
| Genus | Leptocylindrus |
| Species | danicus var. danicus |
| Strain | B650 |
| Clonal | Yes |
| Axenic | No |
| Prelim. NCBI Taxon ID | 163516 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Leptocylindrus danicus var. danicus is a centric colonial diatom branching off deep in the phylogeny of diatoms. It is commonly reported in coastal waters around the world, where it can form intense blooms. Its life cycle is unique among diatoms, as this is the only species in the group that forms resting stages following sexual reproduction. The transcriptome can shed light on the molecular basis of sexual reproduction and auxospore formation in diatoms, and be used as background information in metatranscriptomic investigations during blooms of the species. |
| Additional citations and references | Cleve, P.T., 1889,Pelagisk Diatomeer fr?n Kattegat. InDet Videnskabelige Udbytte af Kanonbaaden "Hauchs" Togter i de Danske Have Indefor Skagen, I. Aarene 1883-86. 53-56; French III, F. W. & Hargraves, P. E. 1985. Spore formation in the life cycles of the diatoms Chaetoceros diadema and Leptocylindrus danicus. Journal of Phycology 21: 477-483; French III, F. W. & Hargraves, P. E. 1986. Population dynamics of the spore-forming diatom Leptocylindrus danicus in Narragansett Bay, Rhode Island. Journal of Phycology 22: 411-20 |
| Environmental Data | |
| Latitude | 40.8083 |
| Longitude | 14.25 |
| Depth (m) | 2 |
| Salinity (psu) | 37.11 |
| Temperature (ºC) | 24.89 |
| Collection date | 15-JUN-10 |
| Sample collection site | Mediterranean Sea |
| Other collection site info | Long Term Ecological Research Station MareChiara (LTER-MC) Gulf of Naples |
| Sample material (e.g. "seawater," "sediment," etc.) | Seawater |
| Chlorophyll (mg/m3) | 2.32 |
| Nirate (μmol/kg) | 0.01 |
| Phosphate (μmol/kg) | 0.02 |
| Dissolved oxygen (ml/L) | 5.77 |
| POC (mg/L) | 0.7 |
| Silicate (μmol/kg) | 1.91 |
| ENVO term for habitat - primary term | Aquatic: marine |
| Habitat description | Coastal waters, Surface layer |
| Habitat | marine habitat |
| Country | ITALY |
| Experimental Data | |
| Date of experiment | 22-APR-11 |
| Growth medium | K |
| Temperature (ºC) | 20 |
| Salinty (psu) | 36 |
| pH | 8.2 |
| Light (µmol photons / m2 / sec) | 100 |
| Day portion of day:night cycle in hours | 12 |
| Night portion of day:night cycle in hours | 12 |
| Nitrate (μmol/L) | 882 |
| Ammonium (μmol/L) | 50 |
| Phosphate (μmol/L) | 36.2 |
| Silicate (μmol/L) | 504 |
| Trace elements (total) (nmol/L) | 22770 |
| Investigation type | Eukaryotes |