Crypthecodinium cohnii Seligo

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Environmental Data
Principle Investigator(s)	Philippe Deschamps
External sample ID	CCs_DP-Rich-G2M
NCGR Sample ID	MMETSP0325_2
Sample accession number	CAM_SMPL_002529
Assembly accession number	CAM_ASM_000347
Combined Assembly Name	Crypthecodinium-cohnii-Seligo
Genus	Crypthecodinium
Species	cohnii
Strain	Seligo
Clonal	Yes
Axenic	Yes
Prelim. NCBI Taxon ID	2866
18S rRNA	AACCTGGTGATCCTGCCAGTAGTCATATGCTTGTCTCAAGATTAAGCCATGCATGTCTGAGTCTATGCCTTTACACGGTTAAGCTGCGAATGGCTCATTATAACAGTAATGATCTATTTGATGATTATCGTTACATGGATAACTACAGTAATTCTGGAGCCAATACATGTTTCAACACCCAACTTATTGGAGGGTGGTGCTTACCAAATTCAGAACCAATCCAAGCTTGCTTGGTTTCATAATGAAGTATGGTAAGAGGATGAATCACATGGTCTTTCTGGTGATGCATCTCATGAGTTTCTGACCTATCAGCTTTGGATGGTAGGGTATTGGCCTACCATGGCAATGACGGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAATGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCTTGACACAAGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTCGTGTTGGAAGGGCGGATTTAAACACTTTGCATGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAATTTCTGCATAGGGCTGTTGGTCCACCCTCTGGGTGTGTATCTGACATGTTCTGTGCATGAGTCTTGATGGCTACAGGGCCTTCGCTGGTCGTGTAGTTGTCAGCTATTTTACTTTGAGGAAATTAGAGTGTTTCAAGCAGGCATGTGCCTTGAATATATTAGCATGGAATAATGAAATTGAGCTTTGGATCTTTTTTATTGGTTTCGAGAACCATCGCAACGATTGATAGGGACAATTGGGGGCATTTGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACACACTAATGCGAAAGCATTTGCCAAGGATGTTTTCTTTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATTAGATACCGTCCTAGTCTTAACCATAAACCATGCCAACTAGAGATTGGGGTGAGTGTCATTATTGCTCTCTCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGATCCAGACATCGGAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTACTAAATAGCTACACATGGCCTAGGCTATGTGGGTAAGCTTCTTAGAGGGACTTTGTATGTTCAATGCAAGGAAGTCTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAGTGTATTTCCTTGCCTGGAAGGGTTGGGTAATCTCTTTAAATTGCATCGTGATGGGGATAGACTCTTGCAATTATTAGTCTTCAACGAGGAATTCCTAGTAAACACGAGTCAACATCTCGTATTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGTGTGCTTCGGTGAATAATTCGGACCGTTCCTCATAAGAGTCTCTCTTTGAGGGGTGGAAAGTTTGGGAACCACAGCACTTGAGGAATGAACGA
Importance of organism and transcriptomes	Crypthecodinium cohnii is a marine heterothrophic dinoflagellate for which several genetic, cytologic and molecular tools were developed, enabling the study of the unique features of the dinoflagellate cell. Culture synchronization and inducible sexual differentiation allows to provide a high range of RNA expression patterns; while functional tools can be developed to test the role of new identified RNA sequences. The evolution history and phylogenetic position of the species into the dinoflagellate group suggests that the endosymbiotic gene content of Crypthecodinium cohnii can provide a new picture of protist evolution especially for photosynthesis acquisition via secondary endosymbiosis and its subsequent loss.
Additional citations and references
Primary citation for organism's characterization, if available	Seligo in Cohn 1186 p.156, Chatton in Grasse 1952 p.347
ENVO term for habitat - primary term	Acquatic: marine
Habitat	marine habitat
Experimental Data
Date of experiment	20-APR-11
Growth medium	MLH (Tuttle & Loeblich, 1975)
Modifications to growth medium	Double Amount of vitamins, 10 g/L glucose
Temperature (ºC)	27
Salinty (psu)	30
pH	7.5
Night portion of day:night cycle in hours	24
Ammonium (μmol/L)	3000
Phosphate (μmol/L)	790
Trace elements (total) (nmol/L)	1800
Investigation type	Eukaryotes
Other experimental metadata available	Synchronized culture, cells harvested in G2 phase (8 hours after synchronization)