Alexandrium minutum CCMP113

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Environmental Data
Principle Investigator(s)	Kjetill Sigurd Jakobsen
External sample ID
NCGR Sample ID	MMETSP0328
Sample accession number	CAM_SMPL_002532
Assembly accession number	CAM_ASM_000350
Combined Assembly Name	N/A
Genus	Alexandrium
Species	minutum
Strain	CCMP113
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	39455
18S rRNA	tacctggttgatcctgccagtagtcatatgcttgtctcaaagattaagccatgcatgtctcagtataagcattcatttggcgaaactgcaaatggctcattaaaacagttataatgcacttgatgatcgattgcttacatggataactgtggtaattctatagctaatacatgcatccaaacctgacttctgggaagggttgtggtcattagttacagaaccaacccaggctctgcttggaattttggtgattcatgatgactgaatgaattacatggcaactgctggtaataaatcattccagtttctgacctatcagcttccgacggtaaggtattggcttaccgtggcaatgacgggtgacggaggattagggtttgattccggagagggagcttgagaaatggctaccacatctaaggaaggcagcaggcgcgcaaattacccaatcctgacacagggaggtagtgacaagaaataacaatacaaggcatccatgtcttgtacttggaatgaatggatattaaacctttctatgagtatcaattggagggcaagtctggtgccagcagccgcggtaattccagctccaatagcgtatattaaagttgttgcggttaaaaagctcgtagttggatttctgctgagggtggctggtccgccctctgggtgagtatctggcacagcctgagcattcttcttgaaagcacaactgcacttgactgtgtggtgtggtattgagaacctttactttgaggaaatcagagtgtttcaagcaggcgtttgccttgaatacattagcatggaataatattatagggccttggttctattttgttggtttctagaactgaggtaatgattaatagggatagttgggggcattcgtatttaattgtcagaggtgaaattcttggatttgttaaagacggactactgcgaaagcatttgccaaggatgttttcattgatcaagaacgaaagttaggggatcgaagacgatcagataccgtcctagtcttaaccataaaccatgccaactagagattggaggtcgttatctctatgacttcttcagcaccttatgagaaatcgaagtctttgggttccggggggagtatggtcgcaaggctgaaacttaaaggaattgacggaagggcaccaccaggagtggagcctgcggcttaatttgactcaacacggggaaacttaccaggtccagacataatgaggattgacagattgatagctttttcttgattctatgggtggtggtgcatggccgttcttagttggtggagtgatttgtctggttaattccgttaacgaacgagaccttaacctgctaaatagttacacgtaattccggttatgtgggcaacttcttagagggactttgtgtgtataacgcaaggaagtttgaggcaataacaggtctgtgatgcccttagatgttctgggctgcacgcgcgctacactgatgtgttcaacgagtttgcaaccttgcctggaaaggttgggtaatcttgaaaaatgcatcgtgatggggattgattattgcaattattaatcttaaacgaggaattcctagtaagcttgagtcatcagcttgtgctgattacgtccctgccctttgtacacaccgcccgtcgctcctaccgattgagtgatccggtgaataattcggactgcagcagtgctcagttcttgaacattgcagtggaaagtttaatgaaccttatcacttagaggaaggagaagtcgtaacaaggtttctgtaggtgaacctgcggaaggatcatttgcacattcctaacaacattaatctttcacgactttgtgagctgtggtggggttcctaggctttaggttctgc
Importance of organism and transcriptomes	Alexandrium minutum is an armoured marine autotrophic dinoflagellate species that occurs in coastal areas around the world. It has two modes of reproduction: it may reproduce asexually by binary fission, which may lead to rapid population growth and dense red-tide coastal blooms, or it may reproduce sexually, which results in the formation of resting cysts. Alexandrium minutum produces potent neurotoxic compounds (saxitoxins) that can have serious deleterious effects on other marine organisms and, upon consumption of vector species, may be fatal to humans.
Additional citations and references
Primary citation for organism's characterization, if available	Halim, Y. (1960). Alexandrium minutum, n. gen. n. sp. dinoflagelle provocant des "eaux rouges". Vie et Milieu 11: 102-105.
Latitude	42.23
Longitude	-8.8
Depth (m)	1
Collection date	01-SEP-87
Sample collection site	OTHER
Other collection site info	Ria da Vigo
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
Habitat	marine habitat
Country	SPAIN
Experimental Data
Date of experiment	20-APR-11
Growth medium	L1
Modifications to growth medium	no modifications
Temperature (ºC)	16
Salinty (psu)	25
pH	8
Light (µmol photons / m2 / sec)	100
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36.2
Silicate (μmol/L)	106
Trace elements (total) (nmol/L)	24571.2
Investigation type	Eukaryotes
Other experimental metadata available	cDNA generated using the dinoflagellate spliced-leader primer DinoSL (Zhang et al. 2007. Spliced leader RNA trans-splicing in dinoflagellates. Proc Natl Acad Sci USA vol. 104 (11) pp. 4618-23)