Scrippsiella hangoei-like SHHI-4

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Environmental Data
Principle Investigator(s)	Karin Rengefors
External sample ID
NCGR Sample ID	MMETSP0367
Sample accession number	CAM_SMPL_002544
Assembly accession number	CAM_ASM_000362
Combined Assembly Name	Scrippsiella-hangoei_like-SHHI_4
Genus	Scrippsiella
Species	hangoei-like
Strain	SHHI-4
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	447022
18S rRNA	ATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCAT GTCTTGTAATTGGAATGAGTAGAATTTTAATCCCTTTATGAGTATCGATT GGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCG TATATNAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGCCGAG GACGACCGGTCCGCCCACTGGGTGTGTATCTGGCTCGGCCTGGGCATCTT CTTGGAGAACGTAGCTGCACTTGATTGTGTGGTGCGGTATCCAGGACTTT TACTTTGAGGAAATTAGAGTGTTTCAAGCAGGCACACGCCTTGAATACAT TAGCATGGAATAATAAGATAGGACCTTGGTTCTATTTTGTTGGTTTCTAG AACTGAGGTAATGATTAATAGGGATAGNTGGGGGCATTCGTATTTAACTG TCAGAGGTGAAATTCTTGGATTTGTTAAAGACGGACTACTGCGAAAGCAT TTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGA CGATCAGATACCGTCCTAGTCTTAACCATAAACCATGCCGACTAGAGATT GGAGGTCGTTATCTATATGACTCCTTCAGCACCTTATGAGAAATCAAAGT CTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATT GACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAAC ACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTGATAG CTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGG AGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAA ATAGTTACACTTAACCTCGGTTAAGTGGGCAACTTCTTAGAGGGACTTTG TGTGTCTAACGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTT AGATGTTCTGGGCTGCACGCGCGCTACACTGATGCGCTCAACGAGTTTAT GACCTTGCCCGATAGGGTTGGGTAATCTTCTTAAAACGCATCGTGATGGG GATAGATTATTGCAATTATTAATCTTCAACGAGGAATTCCTAGTAAGCGC GAGTCATCAGCTCGTGCTGATTACGTCCCTGCCCTTTGTACACACCGCCC GTCGCTCCTACCGATTGAATGATCCGGTGAATAATTCGGACCGCAGCAGT GTTCAGCTCCTGGACGTTGCAG
Importance of organism and transcriptomes	This dinoflagellate is found in brackish & saline lakes in the Vestfold Hills, Antarctica. It has a wide range of salinity tolerance and is a cold-water species. Very similar to Scrippsiella hangoei in the Baltic Sea
Additional citations and references	Logares, R., Daugbjerg, N., Boltovskoy, A., Kremp, A., Laybourn-Parry, J. & Rengefors, K. 2008. Recent evolutionary diversification of a protist lineage. Environmental Microbiology. 10: 1231-1243.
Primary citation for organism's characterization, if available	Rengefors, K., Laybourn-Parry, J., Logares, R., Mashall, W. A., & Hansen, G. 2008. Marine-derived dinoflagellates in Antarctic Saline Lakes: Community Composition and annual dynamics. Journal of Phycology 44 (3):341-349
Latitude	68.46306
Longitude	78.22333
Elevation (m) (above sea level)	8
Salinity (psu)	5
Temperature (ºC)	2
Collection date	01-FEB-05
Sample collection site	OTHER
Other collection site info	Highway Lake
Sample material (e.g. "seawater," "sediment," etc.)	lake water
Filter fraction minimum (μm)	20
ENVO term for habitat - primary term	Aquatic: freshwater
ENVO term for habitat - secondary term	Not Applicable
Habitat description	under ice in saline lake
Habitat	freshwater habitat
Country	ANTARCTICA
Experimental Data
Date of experiment	15-JUN-11
Growth medium	MWC
Modifications to growth medium	Selenium added to a final concentration of 4.55 nmol/L
Temperature (ºC)	3
pH	7
Light (µmol photons / m2 / sec)	20
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	1000
Phosphate (μmol/L)	50
Silicate (μmol/L)	100
Trace elements (total) (nmol/L)	20920
Investigation type	Eukaryotes