Peridinium aciculiferum PAER-2

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Environmental Data
Principle Investigator(s)	Karin Rengefors
External sample ID
NCGR Sample ID	MMETSP0370
Sample accession number	CAM_SMPL_002534
Assembly accession number	CAM_ASM_000352
Combined Assembly Name	Peridinium-aciculiferum-PAER_2
Genus	Peridinium
Species	aciculiferum
Strain	PAER-2
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	268820
18S rRNA	CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCAG TATAAGCTTCTATACGGTGAAACTGCGAATGGCTCATTAAAACAGTTACA GTTTATTTGATGGTCATTCCTTACATGGATAACCGTGGTAATTCTAGAGC TAATACATGCGCCCAAACCCGACTCCGTGGAAGGGTTGTGTTTATTAGTT ACAGAACCAACCCAGGCTCTGCCTGGTCTTGTGGTGATTCATAATAACCG AACGAATCGCATGGCATCAGCTGGCGATGAATCATTCAAGTTTCTGACCT ATCAGCTTCCGACGGTAGGGTATTGGCCTACCGTGGCAATGACGGGTAAC GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC ATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAG GTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGAAT GAGTAGAATTTAAATCCCTTTATGAGTATCGATTGGAGGGCAAGTCTGGT GCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTG CGGTTAAAAAGCTCGTAGTTGGATTTCTGCCGAGGACGACCGGTCCGCCC ACTGGGTGTGTATCTGGCTCGGCCTGGGCATCTTCTTGGAGAACGTAGCT GCACTTGATTGTGTGGTGCGGTATCCAGGACTTTTACTTTGAGGAAATTA GAGTGTTTCAAGCAGGCACACGCCTTGAATACATTAGCATGGAATAATAA GATAGGACCTTGGTTCTATTTTGTTGGTTTCTAGAACTGAGGTAATGATT AATAGGGATAGTTGGGGGCATTCGTATTTAACTGTCAGAGGTGAAATTCT TGGATTTGTTAAAGACGGACTACTGCGAAAGCATTTGCCAAGGATGTTTT CATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCC TAGTCTTAACCATAAACCATGCCGACTAGAGATTGGAGGTCGTTATCTAT ATGACTCCTTCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGG GAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC CAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCA GGTCCAGACATAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTA TGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTT AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACACTTAACC TCGGTTAAGTGGGCAACTTCTTAGAGGGACTTTGTGTGTCTAACGCAAGG AAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGC ACGCGCGCTACACTGATGCGCTCAACGAGTTTATGACCTTGCCCGATAGG GTTGGGTAATCTTCTTAAAACGCATCGTGATGGGGATAGATTATTGCAAT TATTAATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGTG CTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATT GAGTGATCCGGTGAATAATTCGGACCGCAGCAGTGTTCAGCTCCTGGACG TTGCAG
Importance of organism and transcriptomes	This dinoflagellate is found in freshwater lakes but can grow in slightly brackish water. It is a cold water species and is has identical rDNA sequences to Scripsiella hangoei in the Baltic Sea
Additional citations and references	Rengefors, K. & Anderson, D. M. 1998. Environmental and endogenous regulation of cyst germination in two fresh-water dinoflagellates. Journal of Phycology 34 (4): 568?577.
Primary citation for organism's characterization, if available	Logares, R., Daugbjerg, N., Boltovskoy, A., Kremp, A., Laybourn-Parry, J. & Rengefors, K. 2008. Recent evolutionary diversification of a protist lineage. Environmental Microbiology. 10: 1231-1243.
Latitude	18.6
Longitude	59.85
Elevation (m) (above sea level)	15
Temperature (ºC)	2
Collection date	01-FEB-04
Sample collection site	OTHER
Other collection site info	Lake Erken
Sample material (e.g. "seawater," "sediment," etc.)	freshwater
Filter fraction minimum (μm)	20
ENVO term for habitat - primary term	Aquatic: freshwater
ENVO term for habitat - secondary term	Not Applicable
Habitat description	under ice in mesotrophic lake
Habitat	freshwater habitat
Country	SWEDEN
Experimental Data
Date of experiment	15-JUN-11
Growth medium	MWC
Modifications to growth medium	Selenium added to a final concentration of 4.55 nmol/L
Temperature (ºC)	3
pH	7
Light (µmol photons / m2 / sec)	20
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	1000
Phosphate (μmol/L)	50
Silicate (μmol/L)	100
Trace elements (total) (nmol/L)	20920
Investigation type	Eukaryotes